二苯乙烯苷对人脐静脉内皮细胞一氧化氮合酶及其基因的调节作用  

Effects of 2,3,5 4'-tetrahydroxystilbene-2-O-β-D-glucoside on Nitric Oxide Synthase and Gene Expression in Endothelial Cells of Human Umbilical Vein

作　　者：沈建芬[1] 张又枝[2] 肖军花[2] 王嘉陵[2] 

机构地区：[1]浙江省嘉兴市第一医院,314000 [2]华中科技大学同济医学院基础医学院药理系,武汉430030

出　　处：《医药导报》2012年第2期156-159,共4页Herald of Medicine

摘　　要：目的探讨二苯乙烯苷(THSG)对人脐静脉内皮细胞(HUVECs)一氧化氮(NO)含量及内皮型一氧化氮合酶(eNOS)活性的影响及其机制。方法培养HUVECs,分为阴性对照组和THSG 1,10,100μmol.L-1组,用分光光度比色法检测HUVECs上清液中NO含量和eNOS活性,RT-PCR法检测HUVECs eNOS mRNA水平,Western Blot检测HUVECs eNOS蛋白表达。结果与阴性对照组比较,THSG各剂量组HUVECs中NO含量和NOS活性显著提高,eNOSmRNA和蛋白表达上调(P<0.05),并呈剂量依赖性。结论 THSG可通过上调血管内皮细胞中eNOS表达来增加NO量,从而发挥舒张血管作用。Objective To investigate the effects of 2,3,5 4＇-tetrahydroxystilbene-2-O-β-D-glucoside（THSG） on content of nitric oxide（NO） and activity of endothelial nitric oxide synthase（eNOS） and the mechanisms.Methods The human umbilical vein endothelial cells（HUVECs） were cultured and divided into four groups：the control,THSG（1,10,100 μmol·L-1）groups.Spectrophotometry endothelium assay was used to measure NO and activity of eNOS.RT-PCR was used to analyze the eNOS mRNA expression and Western Blot was used to analyze eNOS protein expression level in HUVECs.Results THSG significantly increased the production of NO,activated NOS and up-regulated expression of eNOS mRNA and protein in a concentration-dependent manner compared with the control group.Conclusion THSG raises expression of eNOS mRNA and protein,which may result in activating NO production and relaxing blood vessels.

关 键 词：二苯乙烯苷 人脐静脉内皮细胞 一氧化氮 一氧化氮合酶 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]