高血压增加循环中CXCR4^+细胞而促进新生内膜形成  

Exaggerated neointimal formation in hypertension is associated with increased ciruculating CXCR4^+ cells

作　　者：廖松洁[1] 龚琼[1] 陈健聪[2] 曾意萱[1] 余剑[1] 

机构地区：[1]中山大学附属第一医院神经科,广州510080 [2]中山大学北校区

出　　处：《中国神经精神疾病杂志》2012年第2期92-96,共5页Chinese Journal of Nervous and Mental Diseases

基　　金：国家自然科学基金青年项目(编号:81100871);广东省自然科学基金项目(编号:9451008901002015);广东省卫生厅基金(编号:A2009172);高校基本科研经费青年教师培育项目(编号:09ykpy48)

摘　　要：目的探讨高血压对新生内膜形成的影响及部分可能机制。方法按双肾双夹法复制易卒中型肾血管性大鼠高血压模型(stroke-prone renovascular hypertensive rats,RHRSP),选取收缩压稳定在180 mm Hg以上、无自发性卒中表现的RHRSP,行左侧颈总动脉内膜剥除术。计算颈总动脉内膜、中膜面积和内膜/中膜面积比率。以抗CD31抗体标记内皮细胞检测再内皮化,以抗α-SMA抗体标记血管平滑肌细胞(vascular smoothmuscle cells,VSMCs),以流式细胞仪检测CXCR4+细胞占总单核细胞的百分比。结果对照正常血压组,高血压组的血压显著增高(203.36±17.31 mm Hg;95.5±10.01 mm Hg,P<0.05,n=20),新生内膜面积增加(2.18±0.11 mm2;1.55±0.14 mm2,P=0.013,n=4),循环中CXCR4+细胞百分比增高(损伤前:30.11%±1.27%;20.91%±2.06%,P=0.019;损伤后7 d:45.64%±3.30%;28.28%±2.56%,P=0.003,n=3～5)。高血压不影响再内皮化,新生内膜的主要成分仍为VSMCs。结论高血压增加血管损伤之前和之后早期循环中的CXCR4+细胞,可能与其促进新生内膜形成有关。Objective To investigate the influence of hypertension on carotid neointimal formation and the underlying mechanisms.Methods In this study,stroke-prone renovascular hypertensive rats（RHRSP） were used,and balloon injury was induced in the left carotid artery.Morphological analysis was performed to assess neointimal formation 2 weeks after carotid injury.Re-endothelialization and vascular smooth muscle cells（VSMCs） were detected using CD31 and anti-α-SMA antibodies,respectively.Circulating CXCR4＋ cells were determined by using Fluorescence-activated cell sorting analysis.Results The systolic blood pressure was significantly and persistently increased in RHRSP（203.36 ± 17.31 mm Hg vs 95.5 ± 10.01 mm Hg,P 0.05,n = 20）.Neointimal formation was exaggerated in hypertension（2.18 ± 0.11 mm2 vs 1.55 ± 0.14 mm2,P = 0.013,n = 4）,and circulating CXCR4＋ cell percentage was significantly in creased before and 7 days after carotid balloon injury（before injury： 30.11% ± 1.27% vs 20.91% ± 2.06%,P = 0.019;7 days post injury： 45.64% ± 3.30% vs 28.28% ± 2.56%,P = 0.003,n = 3 ～ 5）.VSMCs were the main components of neointima in hypertension,while re-endothelialization remained identical in both normotension and hypertension.Conclusions This study domenstrates that hypertension enhances neointimal formation and increases circulating CXCR4＋ cells before and early after carotid endothelial denudation,which may contribute to hypertension-induced endothelialization

关 键 词：颈总动脉 新生内膜 高血压 CXCR4 

分 类 号：R544.1[医药卫生—心血管疾病]