猕猴神经干细胞的诱导分化、干性维持及同种脑内移植  

Rhesus monkey embryonic stem cells differentiation,proliferation and allotransplantation

作　　者：董锦润[1,2,3] 郭立云[4] 瞿家桂[1,2,5] 戚仁莉[1,2,5] 王文超[1,2] 肖春杰[3] 王正波[1,2] 

机构地区：[1]中国科学院生物物理研究所脑与认知国家重点实验室,北京100101 [2]中国科学院昆明动物研究所,云南昆明650223 [3]云南大学生命科学院,云南昆明650223 [4]昆明医学院第四附属医院眼科,云南昆明650021 [5]中国科学技术大学生命科学学院,安徽合肥230026

出　　处：《Zoological Research》2012年第1期43-48,共6页动物学研究（英文）

基　　金："973"项目(947703);国家自然科学基金项目(31070963;30670669)

摘　　要：为探索猕猴神经干细胞分化及特性维持,推进神经干细胞临床应用研究,该实验以绿色荧光蛋白(green fluorescence protein,GFP)为标记探讨猕猴胚胎干细胞向玫瑰花环(rosettes)结构神经干细胞的分化及其碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)和表皮生长因子(epidermal growth factor,EGF)的扩增培养。结果表明:1)建立了稳定高效的猕猴神经干细胞分化体系,在该分化体系下,GFP标记猕猴胚胎干细胞在分化的第12天时,95%以上的细胞分化为神经干细胞;2)分化得到的Rosettes结构神经干细胞经bFGF/EGF扩增后,能够较好地维持其Rosettes结构;3)经bFGF/EGF扩增后的rosettes结构神经干细胞移植到猕猴脑内后能够较好的存活并向神经元分化,即bFGF/EGF扩增培养能较好地维持Rosettes结构的神经干细胞,且移植到猕猴脑内的该细胞亦能够较好地存活并向神经元分化,该结果为神经干细胞应用于临床提供了基础理论依据。To investigate the characteristics of rhesus monkey embryonic stem cells and to promote their clinical application,the differentiation and proliferation of rosettes neural stem cells from GFP marked rhesus monkey embryonic stem cells were studied The results showed that：1） A stable and high-efficient neural differentiation system was established.More than 95% of the embryonic stem cells were differentiated into neural stem cells on the 12th days of differentiation;2） the rosettes neural stem cells differentiated from the rhesus monkey embryonic stem cells could maintain their rosettes-shape by proliferating with bFGF/EGF;3） the neural stem cells could differentiate into neurons after transplanted into the rhesus monkey brain.In conclusion,the rosettes neural stem cells differentiated from rhesus monkey embryonic stem cells could maintain their characteristics after proliferation with bFGF/EGF and they could survive and differentiate into neurons after transplanted into the rhesus monkey brain,which strongly supports the clinical application of neural stem cells in the future.

关 键 词：猕猴 神经干细胞 玫瑰花环结构 碱性成纤维细胞生长因子 表皮生长因子 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]