自发性与膳食诱发性食蟹猴2型糖尿病其相关基因的表达比较  

Comparison of gene expression between naturally occurring and diet-induced T2DM in cynomolgus monkeys

作　　者：季芳[1,2] 靳丽莎[1] 曾小明 张秀娟[1] 张艳春 孙云霄[1] 高丽华[3] 何红[4] 饶军华 刘晓明[1,2] 彭白露[1,5] 

机构地区：[1]广东省昆虫研究所,广东广州510260 [2]广东蓝岛生物技术有限公司,广东广州510555 [3]吉林大学第二医院心血管内科吉林长春130041 [4]深圳市福田区慢性病防治院,广东深圳518048 [5]南方医科大学,广东广州510515

出　　处：《Zoological Research》2012年第1期79-84,共6页动物学研究（英文）

基　　金：广州市科技计划项目大型实验动物国际认证及外包服务项目(2009A1-E051);国家"十二五"人类重大疾病灵长类动物模型资源平台的建设(2011ZX09307-303-03)

摘　　要：采用荧光定量PCR技术对自发性和膳食诱发性T2DM食蟹猴外周血白细胞中36个糖尿病相关基因的表达水平进行分析。在36个基因中,糖尿病组的G6PC、CCR2B、CTLA4等19个基因的表达量与对照组相比存在显著差异(P<0.05),且这些基因在诱发组和自发组中的表达模式基本一致。36个基因中,诱发组基因的表达量普遍高于自发组,但大部分基因表达量在两组中差异不显著,表明诱发性和自发性食蟹猴T2DM模型均可作为糖尿病研究较理想的动物模型。因此,通过高能量膳食诱导的食蟹猴糖尿病模型可以替代自发的糖尿病猴,且基因表达量的变化可为疾病的诊断和治疗提供帮助。To explore pathological alteration of T2DM in cynomolgus monkeys,gene expression profiles of peripheral blood leukocytes from spontaneous and diet-induced T2DM models was analyzed using quantitative real-time PCR.Among 36 T2DM associated genes tested,19 genes（including G6PC,CCR2B,CTLA4） displayed a similar expression pattern in both spontaneous and diet-induced T2DM models and were significantly up-regulated or down-regulated compared to controls.Interestingly,expression abundance of all up-regulated genes in the diet-induced T2DM was stronger,although not significantly,than spontaneous models,indicating diet-induced T2DM in monkeys should be a reliable research model for changes in gene expression.The characteristic gene expression pattern obtained here may be useful for the clinical diagnosis of T2DM.

关 键 词：2型糖尿病 基因表达 食蟹猴 

分 类 号：R587.1[医药卫生—内分泌]