明胶微球的制备  

Preparation of gelatin microspheres

作　　者：徐军[1] 陈子扬[1] 单鹏[1] 崔颖杰[1] 马占山 

机构地区：[1]长春生物制品研究所有限责任公司,长春130062 [2]前郭县动物疫病预防控制中心,吉林前郭138000

出　　处：《中国生物制品学杂志》2012年第2期230-232,共3页Chinese Journal of Biologicals

摘　　要：目的初步建立明胶微球制备工艺,并检测微球性质。方法按明胶浓度、pH值、硫酸钠浓度不同配比制备明胶微球,根据成球情况(包括微球均匀度、成球率、碎屑量、粘连现象),筛选微球制备最佳条件。以IFNα2b为模型药物,以最佳条件制备明胶微球,检测IFNα2b活性,并计算包封率。结果在50℃条件下,明胶浓度为40 g/L,调节pH值为3.4和3.6,硫酸钠浓度为300 g/L时,制备的明胶微球较好,平均粒径为18μm,直径范围在15～20μm之间的微球占70%以上,包封率在85%以上。结论所制备的明胶微球可用于缓释注射剂的制备。Objective To preliminarily develop a procedure for preparation of interferon gelatin microspheres （IFN-GMS） and investigate their characteristics. Methods IFN-GMS were prepared, based on which the condition for preparation, such as gelatin concentration, pH value and sodium sulfate concentration, was optimized according to the status of mierospheres including homogene- ity, formation rate, quantity of debris as well as adhesion. The prepared IFN-GMS were determined for IFNα2b activity, based on which the encapsulation rate was calculated. Results The optimal temperature, gelatin concentration, pH value and sodium sulfate conentration for preparation of IFN-GMS were 50℃, 40 g/ L, 3. 4 or 3. 6 and 300 g / L respectively. The microspheres were at a mean diameter of 18 p,m, more than 70% of which were at diameters of 15-20 μm. The encapsulation rate was more than 85%. Conclusion The IFN-GMS may be used for preparation of controlled-released injection.

关 键 词：明胶 微球 迟效制剂 

分 类 号：R392.33[医药卫生—免疫学]