外源腐胺和精胺对NaCl胁迫下草莓叶片膜脂过氧化作用的影响  

Effects of Exogenous Putrescine(Put) and Spermine(Spm) on Membrane-lipid Peroxidation of Strawberry(Fragaria×ananassa) Leaves under NaCl Stress

作　　者：胡淑明[1] 杜强[2] 李青云[1] 张学英[1] 葛会波[1] 

机构地区：[1]河北农业大学,河北保定071001 [2]河北省科技工程学校,河北保定071000

出　　处：《安徽农业科学》2012年第4期1989-1996,共8页Journal of Anhui Agricultural Sciences

基　　金：河北省农业综合开发科技推广项目

摘　　要：[目的]研究不同浓度腐胺(Put)和精胺(Spm)对NaCl胁迫下草莓(Fragaria×ananassa)叶片膜脂过氧化作用的影响,为进一步探讨多胺参与草莓耐盐性反应的作用机理奠定基础。[方法]试验共设8个处理:清水处理(CK),120 mmol/L NaCl,1×10-3mol/L Spm+120 mmol/L NaCl,1×10-4 mol/L Spm+120 mmol/L NaCl,1×10-5 mol/L Spm+120 mmol/L NaCl,1×10-3 mol/L Put+120 mmol/L NaCl,1×10-4 mol/L Put+120 mmol/L NaCl,1×10-5mol/L Put+120 mmol/L NaCl。[结果]腐胺和精胺处理能够降低NaCl胁迫下草莓叶片内O2-.含量、MDA含量和细胞膜透性,提高SOD和POD活性,降低膜脂过氧化水平,提高膜稳定性,进而提高草莓的抗盐性。[结论]Put和Spm能增强植物对盐胁迫的抗性,以1×10-5mol/L Put处理与1×10-3mol/L Spm处理效果最佳。[Objective] The aim was to study the effects of different content of put and Spm on membrane-lipid peroxidation of strawberry leaves under NaCl stress,and lay basis for mechanism of polyamines in strawberry salt resistance.[Method] Eight treatments were designed,which were water（CK）,120 mmol/L NaCl,1×10-3 mol/L Spm＋120 mmol/L NaCl,1×10-4 mol/L Spm＋120 mmol/L NaCl,1×10-5 mol/L Spm＋120 mmol/L NaCl,1×10-3 mol/L Put＋120 mmol/L NaCl,1×10-4 mol/L Put＋120 mmol/L NaCl,1×10-5 mol/L Put＋120 mmol/L NaCl,respectively.[Result] The results showed that the application of exogenous polyamine could reduce the content of O-2· and MDA,decrease the membrane permeability,improve the activities of SOD and POD,debase the membrane-lipid peroxidation level,stabilize the structure of membrane and improve the strawberry salt-resistance ability.[Conclusion] Put and Spm enhance the resistance of plants to NaCl,and the treatment of 1×10-5mol/L Put and 1×10-3mol/L Spm are perfect.

关 键 词：草莓 多胺 盐胁迫 膜脂过氧化 

分 类 号：Q945.78[生物学—植物学]