IFN-γ和TNF-α协同对MIN6细胞凋亡的影响  

Effects of IFN-γ and TNF-α on the apoptosis of insulinoma cells line MIN6

作　　者：董世访[1] 曹朝晖[1] 江伟凡[1] 李桂清[2] 姜曼[2] 胡小波[1] 许桂莲[2] 

机构地区：[1]南华大学生化与分子生物学教研室,衡阳421001 [2]第三军医大学基础部免疫学教研室,重庆400038

出　　处：《免疫学杂志》2012年第3期191-193,199,共4页Immunological Journal

基　　金：国家自然科学基金(30971394)

摘　　要：目的研究细胞因子IFN-γ和TNF-α协同对小鼠胰岛瘤细胞株MIN6凋亡的影响。方法采用四甲基偶氮唑蓝(MTT)比色法观察比较了IFN-γ、TNF-α单独及其组合对MIN6细胞活性的影响,并进一步通过光学显微镜和激光共聚焦显微镜从形态学上对其毒性作用进行了验证,最后通过流式细胞术区分了其对MIN6细胞的凋亡和坏死的作用。结果 IFN-γ和TNF-α单独作用对MIN6细胞活性有轻微影响,但两者组合对MIN6细胞的活性具有显著的抑制作用,并且早期凋亡的细胞百分率明显高于IFN-γ单独处理组(69.91%&13.03%),而坏死或晚期凋亡细胞的百分率两组之间没有差异(2.14%&2.41%)。结论IFN-γ和TNF-α协同可促进MIN6细胞的凋亡。This study is aimed to explore the effects of IFN-γ and TNF-α on the apoptosis of insulinoma cells line MIN6.The cytotoxicity of IFN-γ,TNF-α,or IFN-γ/TNF-α synergism to MIN6 cells was assessed by MTT,and further confirmed morphologically by microscopy and laser confocal microscopy.The apoptotic and necrotic effects of IFN-γ and TNF-α on MIN6 cells were differentiated by FACS followed with Annexin V-FITC/7-aminoactinomycin D（7-AAD） double staining.In contrast to IFN-γ or TNF-α alone,IFN-γ/TNF-α synergism had an obvious inhibition in the viability of MIN6 cells and caused significant apoptosis in MIN6 cells（13.03% and 69.91%）.However,no difference was observed in the percentage of late stage apoptotic and necrotic cells between IFN-γ or TNF-α alone,and IFN-γ/TNF-α synergism（2.41% and 2.14%）.IFN-γ/TNF-α synergism can induce MIN6 cells apoptosis.

关 键 词：IFN-Γ TNF-Α MIN6细胞 凋亡 

分 类 号：R587.1[医药卫生—内分泌]