MicroRNAs途径在RAW264.7细胞中作用的初步研究  

The role of microRNAs pathway in macrophage

作　　者：李佳[1] 张志仁[1] 姜曼[1] 孙怡[1] 傅晓岚[1] 吴玉章[1] 

机构地区：[1]第三军医大学基础部全军免疫学研究所,重庆400038

出　　处：《免疫学杂志》2012年第3期251-254,258,共5页Immunological Journal

基　　金：国家自然科学基金(81070954);第三军医大学校级科研课题(2010xhg04)

摘　　要：目的通过检测microRNAs生成的关键酶Dicer在RAW264.7细胞不同功能状态下的表达差异研究microRNAs途径在其中产生的作用。方法采用QRT-PCR、流式细胞术等对RAW264.7细胞在不同功能分化状态下的Dicer酶进行检测。结果GMCSF与MCSF分别刺激RAW264.7细胞诱导其增殖分化后,Dicer酶的mRNA水平显著增高,但随刺激时间呈现不同趋势(P<0.01);在诱导凋亡死亡状态下,RAW264.7中Dicer酶的表达下降30%(P<0.01);LPS与IL-4分别诱导RAW264.7细胞分化为M1和M2型,Dicer的表达与M1、M2型相关炎性因子的表达有相关性。结论 MicroRNAs调控途径参与并影响RAW264.7不同功能状态。To evaluate the role of microRNAs pathway in macrophage,we detected the expression of Dicer,an important enzyme of microRANs generation in different functional status.Treated with GMCSF and MCSF,RAW264.7 was promoted to proliferation and differentiation,of which Dicer expression detected by real time PCR was increased with different tendency;in the apoptosis and death situation induced by H2O2,Dicer expression was decreased by 30%;macrophages stimulated with LPS assume an M1 proinflammatory phenotype characterized by a high expression level of proinflammatory cytokines such as TNF-α and transcription factors such as C/EBPδ.Dicer expression of M1 detected by real time PCR was decreased while the proinflammatory cytokines TNF-α was increased.In contrast,M2 macrophages were involved in the resolution of inflammation and express different M2 marker genes such as arginase,FIZZ1 and PPARγ,in which phenotype,Dicer expression was increased by 2 times.Above results imply that Dicer-dependent microRNAs pathway maybe participate in and impact various macrophage functions.

关 键 词：MICRORNAS DICER酶 RAW264.7 巨噬细胞 

分 类 号：R392[医药卫生—免疫学]