灵芝富硒栽培研究  

Se-Accumulation Cultivation Technology of Ganoderma lucidum

作　　者：程红艳[1,2] 孙绪春 孟俊龙[4,2] 常明昌[4,2] 冯翠萍[4,2] 

机构地区：[1]山西农业大学资源环境学院,山西太谷030801 [2]山西省食用菌工程技术研究中心,山西太谷030801 [3]山东瑞芝生物科技有限公司,山东梁山272600 [4]山西农业大学食品科学与工程学院,山西太谷030801

出　　处：《安徽农业科学》2012年第5期2626-2627,共2页Journal of Anhui Agricultural Sciences

基　　金：山西省科技攻关项目(20090311047);山西省高校科技开发项目(20081009);山西省科技基础条件平台项目(2010091017)

摘　　要：[目的]研究不同浓度的Na2SeO3对灵芝产量、质量和硒富集量的影响。[方法]采用平板自然选育法对灵芝菌种进行筛选,将筛选出的菌种进行栽培试验,以生物量和菌丝多糖产量为指标,考察不同浓度的Na2SeO3对灵芝产量、质量和硒富集量的影响。[结果]平板筛选结果表明,泰芝一号灵芝的菌丝体生物量最高,多糖产量最高,因此选择泰芝一号作为富硒栽培试验对象;在液体培养液中添加不同浓度的Na2SeO3,可有效促进菌体的生长,但对于菌体多糖的合成则没有明显的促进作用,当添加的Na2SeO3浓度达到0.08%时,菌体生物量达到最大;栽培培养基中添加不同浓度的Na2SeO3对灵芝的生长和多糖的合成均有一定的促进作用;灵芝子实体中硒含量随着Na2SeO3浓度的增加而增加。[结论]栽培培养基中添加Na2SeO3对灵芝子实体的生长及多糖的合成均有一定的促进作用。灵芝子实体中硒含量随着Na2SeO3浓度的增加而增加。[Objective] To investigate on the effects of different concentrations of Na2SeO3 on the yield,quality and Se-accumulation of Ganoderma lucidum.[Methods] Plate natural breeding method was used to select the G.lucidum.Cultivation experiment of the strain selected was conducted.Taking biomass and mycelium polysaccharide yield as the indices,effects of different concentrations of Na2SeO3 on the yield,quality and Se-accumulation of G.lucidum were studied.[Results] Results of pate natural breeding showed that the stain Taizhi No.1 had the greatest biomass and polysaccharide yield.Therefore,Taizhi No.1 was selected as the test object for Se-accumulation.Adding Na2SeO3 in the liquid mediums could effectively promote the growth of thalli,but had no significant promotion on the synthesis of polysaccharide.When the Na2SeO3 concentration reached 0.08%,the biomass yield was the maximum.Adding Na2SeO3 in the cultivation mediums had certain promotion on the growth of G.lucidum and the synthesis of polysaccharide.Se content in fruiting body of G.lucidum enhanced as the concentration of Na2SeO3 increased.[Conclusion] Adding Na2SeO3 in the cultivation mediums could promote the synthesis of polysaccharide and the growth of fruiting body of G.lucidum.Se content in fruiting body of G.lucidum enhanced as the concentration of Na2SeO3 increased.

关 键 词：灵芝[Ganoderma Lucidum(Fr.)Karst] 富硒 生物量 多糖 

分 类 号：S567.3[农业科学—中草药栽培]