不同溶剂索氏提取的白花丹叶中白花丹醌的含量测定  

Content Determination of Plumbagin in the Leaves of Plumbago zeylanica L.by Soxhlet Extraction with Different Solvents

作　　者：杜泽乡[1] 谭宁昌[1] 罗朝晖[1] 

机构地区：[1]桂林医学院,广西桂林541004

出　　处：《安徽农业科学》2012年第5期2628-2629,2639,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]测定不同方法提取的白花丹鲜叶中白花丹醌的含量并加以比较,优选出最佳提取溶剂。[方法]采用索氏提取法,选用不同的溶剂提取白花丹鲜叶中的白花丹醌,并通过HPLC测定其含量,从而确定最佳提取溶剂。[结果]测得用氯仿、石油醚(II)、浓度95%乙醇、无水甲醇提取白花丹醌的含量依次为0.511%、0.341%、0.233%和0.191%;用氯仿提取的白花丹醌含量(提取率)最高。[结论]不同方法提取的白花丹鲜叶中白花丹醌的含量差异明显。[Objective] To determine and compare the contents of plumbagin in the leaves of Plumbago zeylanica L.by different extraction methods,and to select the optimal extraction solvent.[Method] Soxhlet extraction method was adopted;plumbagin in the leaves of P.zeylanica was extracted by different solvents.Its content was detected by HPLC,so as to select the optimal extraction solvent.[Result] Contents of plumbagin extracted by chloroform,petroleum ether（II）,95% ethanol and anhydrous methanol were 0.511%,0.341%,0.233% and 0.191%,respectively.Content of plumbagin in P.zeylanica extracted by chloroform was the highest.[Conclusion] There were significant differences in the content of plumbagin in P.zeylanica fresh leaves by different extraction methods.

关 键 词：白花丹鲜叶 白花丹醌 索氏提取 HPLC 

分 类 号：R284.1[医药卫生—中药学]