车前种质资源遗传多样性ISSR分析  

ISSR Analysis on Genetic Diversity of Plantago asiatica L.Germplasm Resource

作　　者：胡生福[1] 雷俊萍 吴波[3] 

机构地区：[1]江西中医学院药学院,江西南昌330004 [2]江西省医药学校,江西南昌330200 [3]江西中医学院江西省中药种质资源工程技术研究中心,江西南昌330004

出　　处：《安徽农业科学》2012年第5期2681-2683,共3页Journal of Anhui Agricultural Sciences

基　　金：江西省教育厅科技项目(赣教技字[GJJ08331])

摘　　要：[目的]研究江西、湖南、湖北等7个省份的车前种质资源的遗传多样性。[方法]采用ISSR技术,对28份车前样品进行遗传多样性和亲缘关系分析。[结果]从40条引物中共筛选出16条可用于车前遗传多样性分析的ISSR引物,共扩增出131条带,其中多态性条带(PPB)有107条,占81.7%。ISSR数据分析显示,多态性位点百分率(P)为75.3%;平均等位基因观测数为0.071 3;有效等位基因数为0.299 0;Nei,s基因多样性指数(H)为0.360 1;Shannon多态性信息指数(I)为0.535 4。[结论]车前种质资源遗传多样性的地理差异较为明显;野生种与栽培种基因型差异较大。[Objective] To study the genetic diversity of Plantago asiatica L.germplasm resource from seven provinces such as Jiangxi Province,Hunan Province and Hubei Province.[Method] ISSR technology was used to analyze the genetic diversity and genetic relationship of 28 copies of P.asiatica samples.[Result] Sixteen primers that were selected at total forty primers were used to analyze the genetic diversity.131 strips were amplified,among which 107 strips had polymorphism,accounting for 81.7% of the total amount.ISSR data showed that the scale of diversity point（P） was 75.3%,observed number of alleles was 0.071 3,effective number of alleles was 0.299 0,Nei′s gene diversity（H） was 0.360 1,Shannon＇s Information index（I） was 0.535 4.[Conclusion] The genetic diversity of P.asiatica germplasm resource had great difference at geography.The wild varieties and cultivated varieties had great difference at gene type.

关 键 词：车前(Plantago asiatica L.) ISSR 遗传多样性 聚类分析 

分 类 号：Q943[生物学—植物学]