鹅脾脏非特异性转移因子的粗提取与活性鉴定  

Coarse Extraction and Identification of Non-specific Transfer Factors from Goose Spleens

作　　者：蒋小武[1] 曾杨梅[1] 朱乐欣[1] 方志超[1] 宋振辉[1] 

机构地区：[1]西南大学荣昌校区动物医学系,重庆402460

出　　处：《安徽农业科学》2012年第5期2710-2712,共3页Journal of Anhui Agricultural Sciences

基　　金：西南大学荣昌校区大学生创新基金项目;重庆市自然科学基金(2008BB5243)

摘　　要：[目的]从鹅脾脏中提取非特异性转移因子并对其进行活性鉴定。[方法]采用透析法,从鹅脾脏中粗提取转移因子,研究其对机体的免疫力的影响。[结果]获得的鹅非特异性转移因子粗提物澄清透明、微黄色、无毒,不含大分子蛋白质,pH 6.72,小分子多肽含量为23.78μg/ml。雏鸭免疫抗体水平检测试验结果显示:用鸭传染性浆膜炎灭活苗与TF粗提液同时免疫的鸭群抗体滴度显著高于注射生理盐水的对照组。仅注射灭活苗的鸭群抗体滴度与对照组鸭群差异显著。这说明鹅脾脏转移因子粗提液对增强机体的免疫力具有一定作用。[结论]该试验为进一步研究鹅脾脏非特异性转移因子的生物学活性与应用提供理论依据和技术参考。[Objective]To extract non-specific transfer factors（TFs） from goose spleens and to detect the activity of the extracts.[Method]The non-specific TFs were extracted crudely from goose spleens using the dialysis method,and the effects of the extracts on immunity were studied.[Result]The extracts of goose TFs were transparent,light yellow and nontoxic.The extracts were at pH 6.72 and did not contain higher molecular weight proteins.The concentration of minor polypeptides was 23.78 μg/ml.The results of immunization test show that the level of antibodies was higher in the geese inoculated with the inactivated vaccine against infections serositis and the extracts of TFs than in the control geese injected with normal saline.In addition,the level of antibodies was significantly higher in the geese only inoculated with inactivated vaccine than in the control geese.The results show that the extracts of TFs from goose spleens could enhance immunity to a certain extent.[Conclusion]The study provides a theoretical basis and technical reference for further researches on the biological activity of non-specific TFs from goose spleens as well as for application of the extracts.

关 键 词：鹅 转移因子 理化特性 抗体水平 

分 类 号：S811.3[农业科学—畜牧学]