分子印迹胶体阵列检测对硝基苯酚  

Detection of p-Nitrophenol Using Molecularly Imprinted Colloidal Array

作　　者：薛飞[1] 王一飞[1] 王秋鸿[1] 孟子晖[1] 薛敏[1] 黄舒悦[1] 芦薇[1] 

机构地区：[1]北京理工大学化工与环境学院,北京100081

出　　处：《分析化学》2012年第2期218-223,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20775007);863计划(No.2007AA10Z433);环境毒理国家重点实验室开放基金(No.KF2009-08)资助

摘　　要：以对硝基苯酚(p-NP)为印迹模板,丙烯酰胺为功能单体,制备单分散的对硝基苯酚分子印迹胶体微球。通过垂直沉降法将分子印迹胶体微球自组装为分子印迹胶体阵列,采用胶带将分子印迹胶体阵列粘贴固定。固定于胶带上的分子印迹胶体阵列膜显示出良好的稳定性,而且对目标分子p-NP具有明显的光学响应。分子印迹微球吸附目标分子发生溶胀,引起胶体阵列溶涨,分子印迹胶体阵列(MICA)反射峰位置发生移动。实验结果显示,MICA随着p-NP浓度增加,反射峰红移近60 nm,MICA表面颜色由红色逐渐变为蓝紫色;而非印迹胶体阵列红移量只约40 nm。MICA简化了光子晶体凝胶传感材料的制备步骤,为开发新型高性能生化传感器材料提供了新思路。p-Nitrophenol molecularly imprinted colloidal microspheres were prepared by polymerization of functional monomers（acrylamide,AM） and template（p-nitrophenol,p-NP） in poly（methyl methacrylate,PMMA） pre-polymerization solution.Molecular imprinted colloidal array（MICA） was prepared using the molecularly imprinted colloidal microspheres by self-assembly and then fixed by an adhesive tape.The MICA on the adhesive tape is easy to handle and has strong stability.This material senses p-nitrophenol in aqueous solution by shifting reflection wavelength.The adsorption of p-nitrophenol into molecularly imprinted colloidal microspheres would swell the microspheres,thus leading to a reflection peak red shift.The reflection peak red shifted nearly 60 nm when the concentration of p-NP increases from 0 mmol/L to 30 mmol/L,while non-imprinted molecularly imprinted colloidal array（NICA） red shifted about 40 nm.The color of MICA changed from red to blue-violet.This method simplifies the preparation of photonic crystal material which is an efficient substance for developing high performance chemical/biological sensors.

关 键 词：光子晶体 胶体阵列 分子印迹 对硝基苯酚 

分 类 号：O657.72[理学—分析化学]