中药复方益糖康对STZ大鼠PKC蛋白表达的影响  

Effect of Chinese Herbal Compound Yitangkang on PKC Protein Expression of STZ Rats

作　　者：刘鹏[1] 马贤德[1] 王守岩[1] 石岩[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032

出　　处：《辽宁中医杂志》2012年第2期366-367,共2页Liaoning Journal of Traditional Chinese Medicine

基　　金：辽宁省科技厅资助项目(20082063)

摘　　要：目的:探讨中药复方益糖康对STZ大鼠蛋白激酶C(PKC)蛋白表达的影响。方法:采用STZ腹腔注射方法复制糖尿病大鼠模型,随机分为空白对照组、安慰剂组和益糖康组,灌胃6周后,采集大鼠背根神经节(DRG)标本,用免疫组化方法检测其PKC蛋白表达水平。结果:安慰剂组大鼠DRG中PKC蛋白表达水平显著上调,益糖康组较安慰剂组PKC蛋白表达水平显著降低。结论:中药复方益糖康可能抑制PKC激活,是其治疗糖尿病及其并发症的机制之一。Objective ： To explore the effect of Chinese herbal compound Yitangkang on protein kinase C （PKC） expression of STZ rats. Methods ： Diabetic rat model was reproduced by intraperitoneal injection of STZ, randomly divided into control group, the placebo group, and Yitangkang group ,gavaged for 6 weeks ,then the rat dorsal root ganglion （DRG） samples were collected, immunohistochemistry was used to detect the protein levels of PKC. Results ： The placebo group PKC protein levels in DRG were significantly increased, compared with the placebo group, protein levels of PKC in Yitangkang group were significantly reduced. Conclusion：Chinese herbal compound Yitangkang may inhibit PKC activation ,which may be one of the mechanisms of its treatment of diabetes and complications.

关 键 词：糖尿病 背根神经节 蛋白激酶C 免疫组化 

分 类 号：R285.5[医药卫生—中药学]