多氯联苯对斑马鱼胚胎心脏发育的毒性作用  

Study on embryonic toxicity of PCBs in heart development of zebrafish

作　　者：王薛洁[1] 余章斌[1,2] 韩树萍[2] 朱春[2] 彭宇竹[1] 

机构地区：[1]南京医科大学,江苏南京210029 [2]南京医科大学附属南京妇幼保健院儿科,江苏南京210004

出　　处：《临床儿科杂志》2012年第2期168-171,共4页Journal of Clinical Pediatrics

基　　金：国家自然科学基金资助项目(No.30973213)

摘　　要：目的探讨环境污染物多氯联苯(polychlorinated biphenyls,PCBs)对斑马鱼胚胎心脏发育的毒性影响及机制。方法采用水域染毒法,将受精后4 h(4 hours post fertilization,4 hpf)内的斑马鱼胚胎分别暴露于浓度为1 000、500、250、125μg/L的多氯联苯中,作为4个浓度组,然后设置溶剂(0.01%甲醇)对照组、60 mg/L的氯化钠溶液空白对照组。通过死亡率、孵化时间、发育畸形等指标评价多氯联苯对胚胎心脏的发育毒性。结果与对照组相比较,随着PCBs质量浓度的增加,斑马鱼胚胎的孵化率降低,畸形率和死亡率增加。当PCBs达到1 000μg/L时,斑马鱼72 hpf的孵出率由对照组的99.44%下降到75.56%,并且120 hpf的死亡率为40.14%、畸形率为57.78%。结论PCBs能影响斑马鱼胚胎发育,导致斑马鱼胚胎和幼鱼的畸形与死亡。Objective To determine the developmental toxicity of the polychlorinated biphenyls(PCBs)on zebrafish(danio rerio)embryos and to explore its mechanism.Methods The fertilized eggs of zebrafish were exposed to five concentrations of PCBs(1 000 μg/L,500 μg/L,250 μg/L,125 μg/L,0 μg/L)and 0.01% methanol respectively.Results With the increase of PCBs concentrations,the hatching rate of embryos were reduced,while the malformation rate of embryos and the lethality of larvae were increased.When the PCBs concentration reached 1 000 μg/L,the hatching rate of exposed embryos were 75.56%,the malformation rate of embryos were 57.78% at 72 hpf,and the lethality of larvae at 120 hpf were 40.14%.Conclusions PCBs could hamper the development of zebraifsh embryo,and lead to the death and malformation of zebrafish embryo and larvae.

关 键 词：斑马鱼 多氯联苯 心脏发育 

分 类 号：R114[医药卫生—卫生毒理学]