姜黄素对人喉癌细胞株Hep-2增殖和端粒酶活性的影响  

Effects of Curcumin on Proliferation and Telomerase Activity of Hep-2 Cells

作　　者：黄小荣[1] 梁昌盛[1] 骆晓枫[1] 周俊宜[1] 

机构地区：[1]中山大学中山医学院基础医学实验教学中心,广东广州510080

出　　处：《临床医学工程》2012年第2期195-198,共4页Clinical Medicine & Engineering

摘　　要：目的研究姜黄素(curcumin)对人喉癌细胞Hep-2增殖和端粒酶活性表达水平的影响。方法 CCK-8法检测不同浓度姜黄素作用于Hep-2后24h、48h、72h的细胞增殖活性;集落形成试验检测不同浓度姜黄素作用于Hep-2后的细胞增殖抑制率;StretchPCR-银染法检测细胞端粒酶活性变化,RT-PCR分析端粒酶主要组分hTERT的mRNA表达情况。结果①姜黄素对Hep-2细胞的增殖抑制作用具有时间和剂量依赖性。五种浓度(20μmol/L、40μmol/L、60μmol/L、80μmol/L、100μmol/L)姜黄素作用12、24、48、72小时后,IC50分别为81.17μmol/L、40.90μmol/L、33.26μmol/L、31.63μmol/L;②四种浓度(20μmol/L、40μmol/L、60μmol/L、80μmol/L)姜黄素作用于Hep-2细胞48h,一周后各组Hep-2细胞集落形成数和细胞增殖抑制率均呈浓度依赖性,其细胞增殖抑制率与对照组相比,分别为36.25%、57.4%、98.48%、100%;③60μmol/L姜黄素作用于Hep-2细胞24、48、72小时后,与对照组相比,端粒酶活性总光密度值(IOD)分别下降23.19%、60.28%和70.15%,各时间组间有显著性差异(P<0.01);端粒酶逆转录酶(hTERT)mRNA表达水平IOD值分别下降2.90%、58.69%和88.35%,各时间组间有显著性差异(P<0.01)。结论姜黄素可抑制体外培养的Hep-2细胞的增殖活性,下调hTERTmRNA的转录水平并抑制细胞的端粒酶活性,有较好的临床应用前景。Objective To study the effects of curcumin on proliferation, telomerase activity and expression level of telomerase subunits of Hep-2 cells. Methods The proliferation activity of Hep-2 cells was assessed by morphology and CCK-8 assay. Telomerase activity was examined by stretch PCR with sliver staining. The expression status of telomerase reverse transcfiptase （hTERT） was analyzed by reverse transcription-polymerase chain reaction （RT-PCR）. Results （1） Curcumin inhibited the cell viability of Hep-2 cells in a time- and dose-dependent manner with the IC50 of 81.17μmol/L（12 h）, 40.90μmol/L（24 h）, 33.26 μmol/L（48 h）, 31.63 μmol/L（72 h）; （2）The inhibitory rate of cell growth of Hep-2 cells after treated by curcumin at various concentrations for 48 h was 36.25% （20μnol/L）, 57.4% （40 μnol/L）, 98.48% （60μmol/L）, 100%（8μmol/L）; （3） At 24, 48, 72 h after curcumin （60μmol/L） treatment, telomerase activity decreased 23.19%, 60.28% ~l 70.15% respectively with significant differences （P 〈0.01）; expression level of human telomerase reverse transcdptase （hTERT） decreased 2.90% ,58.69% ~ 88.35% respectively with significant differences （P 〈0.01 ）. Conclusion Curcumin can inhibit the proliferation activity of Hep-2 cells cultured in vitro, down-regnlate the transcription level of hTERT mRNA and inhibit the telomerase activity, which has a good prospect for clinical application.

关 键 词：姜黄素 细胞增殖 端粒酶 端粒酶逆转录酶 

分 类 号：R285.5[医药卫生—中药学]