龙麦19 Gli-A1/Glu-A3位点近等基因系低分子麦谷蛋白亚基(LGW-GS)鉴定  

LMW-GS Identification at Gli-A1/GluA3 Loci in NILs of Wheat Variety Longmai 19

作　　者：宋维富[1,2] 赵海滨[1,2] 张延滨[1,2] 李集临[1] 姜焕焕[1,2] 丁琦[1,2] 宋庆杰[2] 于海洋[2] 张春利[2] 辛文利[2] 肖志敏[2] 

机构地区：[1]哈尔滨师范大学生命科学与技术学院,黑龙江哈尔滨150025 [2]黑龙江省农业科学院作物育种研究所,黑龙江哈尔滨150086

出　　处：《黑龙江农业科学》2012年第2期1-5,共5页Heilongjiang Agricultural Sciences

基　　金：国家自然科学基金资助项目(30871525)

摘　　要：为了鉴定小麦品种龙麦19(L-19,2*,7+9,2+12)和一对L-19的Gli-A1位点醇溶蛋白近等基因系L-19-626(2*,7+9,5+10)与L-19-613(2*,7+9,5+10)中与Gli-A1位点紧密连锁的Glu-A3位点低分子麦谷蛋白亚基(LMW-GS),利用一套Glu-A3位点特异低分子麦谷蛋白亚基STS标记对L-19、L-19-613、L-19-626及L-19/L-19-626的F2个体进行PCR扩增。结果表明:在具有醇溶蛋白Gli-A1m的个体中(L-19-626类型),Glu-A3e反应均为阳性,在不具有醇溶蛋白Gli-A1m的个体中(L-19和L-19-613类型),Glu-A3c反应均为阳性。说明编码L-19-626醇溶蛋白的Gli-A1 m基因与编码LMW-GS的Glu-A3e基因紧密连锁,编码L-19和L-19-613的Gli-A1位点醇溶蛋白基因(未命名)与编码LMW-GS的Glu-A3c基因紧密连锁。因此,L-19-626与L-19-613近等基因系品质间的差别很可能是由其LMW-GS Glu-A3e与Glu-A3c基因间的差异引起的。In order to identify LMW-GS at Glu-A3 locus linked with Gli-A1 locus in common wheat variety Longmai 19（L-19,HMW-GS 2＊,7＋9,2＋12）and a pair of NILs（L-19-613 and L-19-626）at Gli-A1 locus with genetic background of Longmai 19 with HMW-GS 5＋10,the materials that L-19,L-19-626 and L-19-613,F2 progeny of L-19/L-19-626 were assayed by a set STS markers of LMW-GS at Glu-A3 locus.The results showed that Glu-A3e amplified specific band in L-19-626 type materials with gliadin Gli-A1m.in L-19 and L-19-613 type materials without gliadin Gli-A1m,Glu-A3c amplified specific band.These results indicated the Gli-A1m at Gli-A1 locus was linked with LGW-GS Glu-A3e at Glu-A3 locus and the undesignated gliadin at Gli-A1 locus in L-19-613 and L-19 were linked with LGW-GS Glu-A3c at Glu-A3 locus.It was possible that quality difference between NILs L-19-626 and L-19-613 was due to LMW-GS gene difference of Glu-A3e and Glu-A3c at Glu-A3 locus.

关 键 词：低分子麦谷蛋白亚基 近等基因系 STS标记 

分 类 号：S512.1[农业科学—作物学]