烟草烟雾暴露大鼠中CD4^＋白细胞介素-17^＋T细胞与CD4^＋Foxp3^＋调节性T细胞的变化及意义  

作　　者：蒙菁菁[1] 钟小宁[1] 白晶[1] 何志义[1] 张建全[1] 黄秋嫔[1] 

机构地区：[1]广西医科大学第一附属医院呼吸内科,南宁530021

出　　处：《中华结核和呼吸杂志》2012年第1期55-60,共6页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：国家自然科学基金（30860106）广西壮族自治区人民医院蓝娇医生提供流式细胞仪并帮助检测

摘　　要：目的观察烟草烟雾暴露大鼠外周血与BALF中CD4白细胞介素（IL）-17^＋T细胞（Th17细胞）与CO4^＋Foxp^3＋调节性T细胞（Treg细胞）及相关因子的水平变化，探讨Th17和Treg细胞在烟草诱导气道炎症和COPD的发生与发展中的作用。方法将40只健康清洁级雄性Wistar大鼠分为暴露12周组和24周组、对照12周组和24周组，每组10只。用烟熏法复制大鼠气道炎症的动物模型。收集BALF进行细胞学计数和分类，采用酶联免疫吸附法检测大鼠血清和BALF上清液中IL-17和IL-6水平，用流式细胞术检测Th17和Treg细胞比例，用实时荧光定量PCR法检测IL-17和Foxp3mRNA的表达。多组间比较采用单因素方差分析，组内两两比较采用SNK法和Games—Howell法。结果暴露12周组和24周组大鼠外周血IL-17浓度分别为（52．6±1．8）ng／L和（75．4±6．0）mg／L，BALF中IL-17浓度分别为（78．1±5．8）ng／L和（95．0±6．8）ng／L，均显著高于对照12周组[（40．0±3．2）,lg／L和（54．5±4．6）．g／L]及24周组[（36．7±3．2）ng／L和（53．9±3．7）ng／L]，暴露24周组与其他3组比较，差异均有统计学意义（均P〈0．05）。暴露24周组大鼠外周血中IL-6浓度为（31．4±2．1）,lg／L，显著高于对照24周组[（11．5±0．5）ng／L]；暴露12周组和24周组大鼠BALF中IL-6浓度分别为（33．3±2．3）ng／L和（44．6±3．0）ng／L，显著高于对照12周组和24周组[（15．6±1．8）ng／L和（18．0±1．9）ng／L]。暴露12周组和24周组大鼠外周血中Th17细胞比例分别为（1．81±0．19）％和（3．74±0．55）％，BALF中Th17细胞比例分别为（7．84±0．28）％和（8．01±0．39）％，均显著高于对照12周组[（0．97±0．08）％和（5．64±0．54）％]及24周组[（1．08±0．10）％和（5．95±0．48）％]，暴露24周组与其他3组比较，差异均有统计学意义（均P〈0．05）。暴露12周组和24周组大�Objective To evaluate the changes of CD+4 IL-17 + T ( Th17 ) and CD+4 Foxp3 +regulatory T (Treg) cells in peripheral blood and bronchoalveolar lavage fluid (BALF),and therefore to explore the role of Th17 and Treg in cigarette smoke-induced airway inflammation/COPD in rats.Methods Forty male Wistar rats were randomly divided into 4 groups: a 12 wk smoke-exposure group,a 24 wk smokeexposure group,a 12 wk control group and a 24 wk control group (n =10 each). Cells in BALF were collected and analyzed by absolute and differential cell counts.IL-17 and IL-6 levels in serum and BALF were tested by emzyme linked immunosorbent assay (ELISA).The proportion of CD4+ IL-17 + T and CD4+Foxp3 + Treg in peripheral blood and BALF were determined by flow cytometry.The mRNA expressions of IL-17 and Foxp3 were measured by real-time PCR.Comparisons of the data between different groups were performed using one-way ANOVA,and SNK and Games-Howell test were used for comparison between 2 groups.Results Levels of IL-17 were remarkable increased in the 12 wk smoke-exposure group and the 24 wk smoke-exposure group in serum [ (52.6 ± 1.8) ng/L,(75.4 ± 6.0) ng/L] and BALF [ (78.1 ±5.8) ng/L,(95.0 ±6.8) ng/L] compared with the 12 wk control group [ (40.0 ± 3.2) ng/L,(54.5 ±4.6) ng/L] and the 24 wk control group [ (36.7 ±3.2) ng/L,(53.9 ±3.7) ng/L],all P ＜0.05.IL-6in serum was significantly increased in the 24 wk smoke-exposure group [ (31.4 ± 2.1 ) ng/L] compared with the 24 wk control group [ (11.5 ± 0.5) ng/L],and it was increased in the 12 wk and the 24 wk smoke-exposure group [ (33.3 ±2.3) ng/L,(44.6 ±3.0) ng/L] compared with the 12 wk and the 24 wk control group [ ( 15.6 ± 1.8) ng/L,( 18.0 ± 1.9) ng/L] in BALF.Ratio of Th17 was higher in the 12 wk and the 24 wk smoke-exposure groups in peripheral blood [ ( 1.81 ± 0.19 ) ％,( 3.74 ± 0.55 ) ％ ] and BALF [(7.84±0.28)％,(8.01 ±0.39)％] compared with the12 wk [(0.97 ±0.08)％,(5.64 ±0.54 ) ％ ] and the 24 wk control group [ ( 1.08 ± 0.10) ％,(5.95 ± 0.48 ) ％ ].

关 键 词：CD4 阳性T淋巴细胞 T淋巴细胞 调节性 烟草 炎症 

分 类 号：R563.9[医药卫生—呼吸系统]