系统性红斑狼疮小鼠肾组织γδT细胞TGF-β1表达研究  

Production of TGF-β1 by γδT Cells in the Kidneys of MRL/lpr Mice with Systemic Lupus Erythematosus

作　　者：程军[1] 张雯[1] 程擎天[1] 羊淑敏[1] 

机构地区：[1]杭州市红十字会医院肾内科,杭州310003

出　　处：《浙江中西医结合杂志》2012年第2期89-91,共3页Zhejiang Journal of Integrated Traditional Chinese and Western Medicine

基　　金：杭州市科技局资助项目(No.2006533Q18)

摘　　要：目的:研究系统性红斑狼疮(SLE)MRL/lpr小鼠肾组织γδT淋巴细胞分泌细胞因子TGF-β1的功能。方法:流式细胞仪分析MRL/lpr小鼠外周血、肾组织γδT淋巴细胞比例,并体外选择性扩增γδT细胞,在体外培养条件下,测定培养体系中细胞因子TGF-β1的表达。结果:MRL/lpr小鼠外周血、肾组织中γδT淋巴细胞比例升高,肾组织中γδT淋巴细胞能异常分泌TGF-β1细胞因子。结论:肾组织γδT细胞可能通过分泌TGF-β1在狼疮性肾炎发病中起着免疫调节的保护性功能。Objective：To investigate the production of TGF-β1 by stimulated γδT cells in the kidneys from MRL/lpr mice with systemic lupus erythematosus.Methods：The proportion of γδT cells in peripheral blood and renal tissues in MRL/lpr mice were measured by flow cytometry.Then γδT cells was expanded in vitro to detemine the level of active TGF-β1 in supernatants by ELISA.Results：The proportion ofγδ T cells in peripheral blood mononuclear cells and the renal tissues from MRL/Ipr mice was significantly higher than those in the controls.The level of TGF-β1 produced by activated γδT cells in renal tissues from MRL/Ipr mice was significantly higher than that from the controls.Conclusions：γδT cells may play an important role in the pathogenesis of systemic lupus erythematosus via production of TGF-β1.

关 键 词：小鼠 系统性红斑狼疮 TCRγδ细胞 转化生长因子Β1 

分 类 号：R593.241[医药卫生—内科学]