长期递增负荷运动对小肠集合淋巴结结构及淋巴细胞凋亡的影响  

Effects of exercising with a chronically increasing load on the structure and lymphocyte apoptosis of Peyer's patches

作　　者：覃飞[1] 郝选明[1] 

机构地区：[1]华南师范大学体育学院,广东广州510006

出　　处：《体育学刊》2012年第1期134-138,共5页Journal of Physical Education

基　　金：广东省自然科学基金(9151063101000059)

摘　　要：观察6周递增负荷运动中,大鼠小肠集合淋巴结（PP结）形态结构及淋巴细胞凋亡的基本特征。雄性SD大鼠64只,运动组进行6周递增负荷跑台运动,分别在第0、2、4、6周末观察小肠PP结个数和PP结形态学变化,并通过淋巴细胞Bax、Bcl-2表达和SOD、MDA水平探讨对淋巴细胞凋亡的影响。结果表明：1）PP结数目呈现先下降后回升的变化趋势。2）PP结结构改变主要发生在生发中心及拱顶区（B cell区）,第2~4周PP结生发中心出现断裂带,拱顶顶部出现空泡,第6周有所恢复。3）Bax与Bcl-2比值升高,提示淋巴细胞凋亡增多,且主要发生在PP结生发中心。4）运动应激性自由基生成可能是肠道淋巴结细胞凋亡的机制之一。In order to observe the basic characteristics of the morphological structure and lymphocyte apoptosis of Peyer’s patches of rats during 6-week exercising with an increasing load,the authors divided 64 male SD rats into an exercise group and a control group,put the rats in the exercise group on a treadmill for 6-week exercising with an increasing load,observed the change of the number of Peyer’s patches and the morphological change of Peyer’s patches at the end of weeks 0,2,4 and 6 respectively,and probed into the effects of the expressions of lymphocytes Bax and Bcl-2 and the levels of SOD and MDA on lymphocyte apoptosis.The authors revealed the following find-ings： 1） the number of Peyer’s patches showed a trend of decreasing followed by increasing;2） the change of the structure of Peyer’s patches mainly occurred in the germinal center and the dome zone（B cell zone）;in weeks 2~4,rupturing belts appeared in the germinal center of Peyer’s patches,bubbles appeared at the top of the dome,the con-ditions were somewhat restored in week 6;3） the ratio of Bax to Bcl-2 increased,which suggested that lymphocyte apoptosis increased,and mainly occurred in the germinal center of Peyer’s patches;4） the generation of free radicals induced by exercising may be one of the mechanisms of intestinal lymphocyte apoptosis.

关 键 词：运动生物化学 运动免疫 免疫组织化学 小肠集合淋巴结 淋巴细胞凋亡 自由基 

分 类 号：G804.7[文化科学—运动人体科学]