HPLC法测定丙酸氟替卡松乳膏中丙酸氟替卡松的含量  

Determintion of fluticasone propionate in fluticasone propionate cream by HPLC

作　　者：赵艳[1] 张虹[2] 张霄翔[1] 

机构地区：[1]合肥工业大学制药工程系,安徽合肥230009 [2]安徽省医药科技实业公司,安徽合肥230001

出　　处：《安徽医药》2012年第1期33-35,共3页Anhui Medical and Pharmaceutical Journal

摘　　要：目的建立丙酸氟替卡松乳膏中丙酸氟替卡松含量测定的方法。方法采用高效液相色谱法,色谱柱为C18柱(250 mm×4.6 mm,5μm),流动相为0.02 M磷酸溶液(三乙胺调pH=4.5)-甲醇(24∶76),流速1.0 ml.min-1,检测波长为240 nm,柱温35℃。结果丙酸氟替卡松进样浓度在10～35 mg.L-1范围内与峰面积呈良好线性关系(r=0.999 9),平均回收率为98.83%,RSD为0.47%(n=9)。结论测定方法专属性强、精密、准确、灵敏、简便可行,可作为丙酸氟替卡松乳膏的质量控制方法。Objective To establish a method for the determination of fluticasone propionate in fluticasone propionate cream.Methods The C18 column was used with the mobile phase of 0.02 M phosphoric acid solution（adjusted to pH=4.5 with triethylamine）-methanol（24∶ 76） and with the flow rate of 1.0 ml·min-1 at the detection wavelength of 240nm.The detection temperature was 35℃.Results The calibration curve for fluticasone propionate was linear in the concentration ranges of 10~35 mg·L-1（r=0.999 9）.The average recovery was 98.83%,and RSD was 0.47%（n=9）.Conclusion This assay is specific,precise,accurate,sensitive and could be used as the method of quality control of futicasone propionate cream.

关 键 词：丙酸氟替卡松乳膏 丙酸氟替卡松 高效液相色谱法 含量测定 

分 类 号：R927[医药卫生—药学]