HPLC测定伤湿止痛巴布剂中阿托品的含量  

Determination of Atropine in Shangshizhitong Cataplasm by HPLC

作　　者：贺成[1] 杜守颖[2] 吴笑如[1] 王乃婕[1] 田瑞华 

机构地区：[1]中药复方新药开发国家工程研究中心,北京100075 [2]北京中医药大学,北京100102 [3]中国北京同仁堂(集团)有限责任公司,北京100075

出　　处：《中国中医药信息杂志》2012年第2期53-54,共2页Chinese Journal of Information on Traditional Chinese Medicine

基　　金："十一五"国家科技支撑计划(2008BAI53B077)

摘　　要：目的建立伤湿止痛巴布剂中阿托品的含量测定方法。方法运用高效液相色谱法,Apollo-C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-磷酸盐缓冲液(12∶88)为流动相,流速为1.0 mL/min,检测波长为210 nm。结果阿托品在0.3152～3.94μg范围内线性关系良好(r=0.99995),平均加样回收率为100.7%,RSD=2.2%。结论本法简便、准确、重复性好、专属性强,为伤湿止痛巴布剂质量控制提供了方法。Objective To establish a method for the determination of atropine in Shangshizhitong Cataplasm.Methods HPLC was used with Apollo-C18 column（4.6 mm×250 mm,5 μm）,acetonitrile-phosphate buffer（12∶88） as mobile phase,the flow rate at 1.0 mL/min and detected wavelength set at 210 nm.Results Atropine showed a good linearity relationship in the range of 0.315 2-3.94 μg,r=0.999 95.The average recovery was 100.7%,RSD was 2.2%.Conclusion The method is simple and accurate,with good reproducibility and strong specificity.It can be used for the quality control of Shangshizhitong Cataplasm.

关 键 词：伤湿止痛巴布剂 阿托品 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]