钌(Ⅱ)配合物合成、表征与DNA相互作用及其抗氧化性研究  

Synthesis,Characterization,DNA-binding and Antioxidant Activity Studies of Ruthenium(Ⅱ) Complex

作　　者：徐丽[1] 何娟[1] 闻伴康[1] 罗简胜[1] 潘堪尚[1] 

机构地区：[1]广东药学院医药化工学院,广东中山528458

出　　处：《中山大学学报（自然科学版）》2012年第1期72-75,81,共5页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东药学院师资队伍建设经费资助项目;广东药学院大学生创新实验资助项目

摘　　要：设计合成一个新的钌(Ⅱ)多吡啶配合物[Ru(dip)2(DBHIP)](ClO4)2{dip=4,7-二苯基-1,10-邻菲咯啉;DBHIP=2-(3,5-二溴-4-羟基苯)并咪唑[4,4-f]-(1,10-邻菲啰啉)},采用元素分析,质谱和1H NMR对其进行表征。用电子吸收光谱、黏度测试、Job-plot荧光滴定法研究配合物与CT DNA作用,结果表明配合物以经典的插入模式与DNA键合。采用琼脂糖凝胶电泳实验研究配合物诱导pBR322DNA断裂。同时也研究配合物在高浓度情况下使pGL3 DNA缩合。A novel ruthenium（Ⅱ） polypyridyl complex [Ru（dip）2（DBHIP）]（ClO4）2（dip = 4,7-diphenyl-1,10-phenanthroline,DBHIP = 2-（3,5-dibromo-4-hydroxyphenyl）imidazo phenanthroline） was synthesized and characterized.The DNA-binding property of the complex was investigated by spectroscopic methods and viscosity measurement.The results indicated that the complex interacted with DNA through intercalative mode.The photocleavage of pBR322 DNA by Ru（Ⅱ） complex was investigated.The complex induced the aggregation of plasmid pGL3 DNA by Gel retardation assay.The antioxidant activity of the ligand and the complex was also performed.

关 键 词：钌(Ⅱ)配合物 DNA缩合 DNA键合 

分 类 号：O627[理学—有机化学]