土荆皮抗真菌化学成分研究  

Antifungal constituents of Pseudolaricis Cortex

作　　者：徐云辉[1] 张帅[2] 张念[1] 孔德云[1] 华茉莉[1] 

机构地区：[1]上海医药工业研究院创新药物与制药工艺国家重点实验室,上海200040 [2]郑州市儿童医院,河南郑州450053

出　　处：《中草药》2012年第2期220-222,共3页Chinese Traditional and Herbal Drugs

基　　金：国家"重大新药创制"科技重大专项资助项目(2009ZX09301-007);上海市科学技术委员会国际科技合作基金项目(09540704500)

摘　　要：目的对土荆皮Pseudolaricis Cortex抗真菌活性部位进行化学成分研究。方法土荆皮粗粉以95%乙醇回流提取,所得浸膏采用溶剂法分为不同提取部位,体外抗真菌活性测试显示其醋酸乙酯部位对白色念珠菌具有显著的抑制作用。采用硅胶、Sephadex LH-20、制备高效液相色谱对土荆皮醋酸乙酯部位进行分离纯化,并通过波谱数据分析对化合物进行结构鉴定。结果从土荆皮醋酸乙酯部位分离得到5个化合物,分别鉴定为土荆皮酸B(1)、土荆皮酸C(2)、香草酸(3)、香草酸-4-O-β-D-阿洛糖苷(4)、17-羟基土荆皮酸B(5)。结论化合物5为一新化合物,命名为土荆皮酸I;化合物4为首次从该植物中分离得到。体外抗真菌活性测试显示,化合物1对白色念珠菌生长具有显著的抑制作用,化合物2和5仅显示微弱的抑菌活性。Objective To study the chemical constituents from Pseudolaricis Cortex with antifungal activities. Methods The crude powder of Pseudolaricis Cortex was refluxed with 95% ethanol and different fractions were obtained with different solvants. In vitro antifungal results revealed that EtOAc fraction showed inhibitory effects on Candida albicans. Compounds were isolated by column chromatography on silica gel, Sephadex LH-20, and preparative HPLC from EtOAc ~raction of Pseudolaricis Cortex and their structures were elucidated mainly by spectral data. Results Five compounds were identified as pseudolaric acid B （1）, pseudolaric acid C （2）, vanillic acid （3）, vanillic acid-4-O-β-D-allopyranoside （4）, and 17-hydroxypseudolaric acid B （5）, respectively. Conclusion Compound 5 is a new compound named pseudolaric acid I, and compound 4 is isolated from this plant for the first time. Compound 1 shows stronger activity against the growth of Candida albicans, while compounds 2 and 5 have weak antifungal activities in vitro.

关 键 词：土荆皮 土荆皮酸I 土荆皮酸B 香草酸4-O-β-D-阿洛糖苷 抗真菌 

分 类 号：R284.1[医药卫生—中药学]