基于抗菌效价检测的板蓝根颗粒制备过程质量变化评价  

Evaluation on quality change of Isatidis Radix Granule during preparation process based on antibacterial potency

作　　者：孙琴[1,2] 李寒冰[2,3] 鄢丹[2] 李远[2,4] 胡晓燕[1] 姜黎[1] 肖小河[2] 

机构地区：[1]泸州医学院药学院,四川泸州646000 [2]解放军302医院全军中药研究所,北京100039 [3]河南中医学院,河南郑州450008 [4]河北大学中医学院,河北保定071000

出　　处：《中草药》2012年第2期259-264,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金面上项目(30873381);国家杰出青年科学基金项目(30625042)

摘　　要：目的对板蓝根颗粒制备过程中抗菌活性及化学成分的变化进行评价。方法按照《中国药典》2010年版收载的板蓝根颗粒制备工艺,分别对药材→水提浓缩→醇沉→干燥这4个制备环节所得样品进行化学分析,并采用管碟法检测各样品的抗菌效价。结果板蓝根颗粒在4个环节的制备过程中,成品的化学指纹图谱共有峰数下降了32%,腺苷、尿苷的量也呈递减趋势,同时抗菌效价也下降了60%。结论目前板蓝根颗粒在满足纯化、成型工艺要求的过程中,药效可能损失较大,应以药效或效价作指导,加强其制备工艺的合理性研究。Objective To study the component and quality changes in the preparation process oflsatidis Radix Granule. Methods According to the preparation method recorded by Chinese Pharmacopoeia, the samples via water extracted, concentrated, precipitated by ethanol, and dried were analyzed. The antibacterial effect was detected by cylinder-plate method. Results After preparation, the common peaks in the fingerprint chromatograms oflsatidis Radix Granule were decreased by 32% and the contents of adenosine and uridine were also declined. The antimicrobial potency of Isatidis Radix Granule was decreased by 60%. Conclusion During the preparation process, the antimicrobial potency oflsatidis Radix Granule is declined greatly. So the preparation technique oflsatidis Radix Granule should be guided by it＇s pharmacodynamic action or potency.

关 键 词：板蓝根颗粒 抗菌效价 制备工艺 腺苷 尿苷 指纹图谱 

分 类 号：R284.2[医药卫生—中药学]