草苁蓉环烯醚萜苷对H_(22)小鼠肝癌移植瘤的抑瘤作用  

Anti-tumor effect of iridoid glucosides from Boschniakia rossica in H_(22)-bearing mice

作　　者：金爱花[1] 朴龙[2] 尹学哲[2] 全吉淑[3] 

机构地区：[1]延边大学附属医院检验科,吉林延吉133000 [2]延边大学附属医院内科学教研室,吉林延吉133000 [3]延边大学基础医学院生物化学与分子生物学教研室,吉林延吉133000

出　　处：《中草药》2012年第2期332-335,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30360113);吉林省科技发展计划资助项目(200705426)

摘　　要：目的探讨草苁蓉环烯醚萜苷对小鼠H22移植瘤的生长抑制作用。方法建立小鼠皮下H22移植瘤模型,将实验动物分为模型组、草苁蓉环烯醚萜苷高、中、低剂量(400、200、100 mg/kg)组和5-Fu(25 mg/kg)组,草苁蓉环烯醚萜苷组连续ig给药10 d;5-Fu组隔日ip给药,给药5次,计算抑瘤率、胸腺指数和脾脏指数,并以ELISA法检测血清肿瘤坏死因子-α(TNF-α)和白细胞介素-2(IL-2),比色法检测血清总抗氧化活力(T-AOC)和丙二醛(MDA)水平。结果与模型组比较,草苁蓉环烯醚萜苷能显著减轻移植瘤瘤质量,高、中、低剂量组抑瘤率分别为38.05%、34.98%、26.95%。同时,明显升高荷瘤小鼠的脾脏指数,升高血清IL-2水平和降低TNF-α水平,升高血清T-AOC和降低MDA水平。结论草苁蓉环烯醚萜苷对H22移植瘤具有明显的抑制作用,其作用机制可能与调节IL-2和TNF-α等细胞因子的表达以及增强荷瘤小鼠抗氧化能力有关。Objective To investigate the anti-tumor effect ofiridoid glucosides from Boschniakia rossica （IGBR） in H22-bearing mice. Methods The H22 transplanted mouse models were established and randomly divided into five groups： model, high-, medium- and low-dose IGBR, and 5-Fu groups. Mice were ig treated with 400, 200, and 100 mg/kg IGBR once daily for 10 d, and with ip 5-Fu 25 mg/kg every other day for five times, then the inhibition ratios of tumor growth and indexes of spleen and thymus were caluclated. The serum tumor necrosis factor-α （TNF-α） and interlekin-2 （IL-2） were determined with ELISA method. The total anti-oxidant capacity （T-AOC） and malondialdehyde （MDA） level were detected by colorimetric method. Results The administration with 400, 200, and 100 mg/kg.d IGBR inhibited the growth of transplanted tumor with the inhibitory ratios of 38.05%, 34.98%, and 26.95%, respectively. In addition, IGBR treatment elevated the spleen index and serum IL-2 level, reduced the serum TNF-α level, increased the serum T-AOC, and decreased serum MDA level of H22-bearing mice. Conclusion IGBR could exhibit an inhibitory effect on the growth of transplanted H22 tumor, probably via the regulation of IL-2 and TNF-α expression as well as improvement of anti-oxidant capability of HE2-bearing mice.

关 键 词：草苁蓉 环烯醚萜苷 H22 抗肿瘤 抗氧化 

分 类 号：R285.5[医药卫生—中药学]