没药的质量分析与评价  

Quality analysis and evaluation of myrrh

作　　者：宿树兰[1] 蒋海峰[1] 严辉[1] 钱大玮[1] 段金廒[1] 唐于平[1] 

机构地区：[1]南京中医药大学药学院,江苏南京210046

出　　处：《中草药》2012年第2期364-371,共8页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30973885);江苏省高校自然基金重大基础研究资助项目(06KJA36022);江苏省方剂高技术研究重点实验室建设项目资助(BM2010576);江苏省理血方剂创新药物工程中心建设资助项目;江苏高等学校优秀科技创新团队支持计划(2009年度)

摘　　要：目的建立客观评价没药药材质量的分析方法,对收集的不同批次没药药材的质量进行分析与综合评价,为其质量控制提供参考。方法采用GC-MS法对没药中挥发性成分进行分析与鉴定;采用HPLC-PDA法分析没药中非挥发性成分;并采用相似度软件和SPSS 16.0软件进行相似度评价和主成分分析。结果 GC-MS法分析鉴定了没药中28个化学成分,建立了不同批次没药药材非挥发性成分的HPLC特征图谱;两类指纹图谱相似度分析结果均表明,收集于不同批次的没药药材相似性较差,其中收集于海南和广东的相似度较高;主成分分析结果表明倍半萜烯类成分、有机酸酯类成分和二萜酸类成分对药材品质起到主导作用。综合分析提示,收集于海南药材公司的没药药材质量较佳。结论所建立的质量分析方法为没药质量控制提供了科学依据。Objective To establish the methods for the quality evaluation of Commiphora myrrha in different batches and provide the reference for quality control. Methods GC-MS and HPLC-PDA were used to analyze the volatile and non-volatile components, respectively. Similarity and principle components analysis （PCA） were carried out. Results Twenty-eight compounds were identified by GC-MS from C. myrrha and the HPLC fingerprint of non-volatile components was established. Results of similarity analysis showed that C. myrrha in different batches was different from each other except for batches from Hainan and Guangdong with high similarity. PCA demonstrated that sesquiterpenes, organic acid ester, and diterpenic acids played a leading role in the quality of C. myrrha. All results revealed that C. myrrha from Hainan had high qualities. Conclusion The established method could provide the guidance for quality control of C. myrrha.

关 键 词：没药 GC—MS HPLC—PDA 品质评价 主成分分析 

分 类 号：R286.02[医药卫生—中药学]