不同生态环境下稻米淀粉RVA谱特征值的QTL定位分析  

Identification of QTL for Rice Starch RVA Profile Properties under Different Ecological Sites

作　　者：杨亚春[1] 倪大虎[1] 宋丰顺[1] 李莉[1] 陆徐忠[1] 李泽福[1] 杨剑波[1] 

机构地区：[1]安徽省农业科学院水稻研究所,安徽合肥230031

出　　处：《作物学报》2012年第2期264-274,共11页Acta Agronomica Sinica

基　　金：国家转基因生物新品种培育重大专项(2009ZX08001-13B);国家自然科学基金项目(31071401);国际合作项目(2008DFA31840)资助

摘　　要：利用2个直链淀粉含量相似的水稻品种9311和日本晴为亲本,采取单粒传法创建由190个家系组成的重组自交系群体,并构建了包含202个SSR、CAPs和STS标记的遗传连锁图谱。采用复合区间作图法,在3个不同生态环境下(海南陵水、安徽合肥和怀远)对RVA谱特征值(峰值黏度、热浆黏度、崩解值、冷胶黏度、消减值、峰值时间、起浆温度和回复值)的8个特征性状进行了QTL分析。共定位到57个QTL,单个性状QTL数目在1～14个之间,说明RVA谱特征值是多基因控制的数量性状。13个QTL在3个不同环境中被2次或3次检测到,其中qCPV-3、qCPV-10b、qSBV-10b、qCSV-3b、qCSV-10b(贡献率分别为26.9%、29.5%、29.7%、25.2%、28.3%)被3次检测到,稳定性较高。16个QTL具有一因多效性,单个QTL位点控制的性状一般在2～6个之间,第10染色体RM25032～RM1375区段控制峰值黏度、热浆黏度、冷胶黏度、消减值、峰值时间和回复值等6个性状。Two rice varieties with similar apparent amylose content,Nipponbare（japonica） and 9311（indica）,were used as par-ents to establish a recombinant inbred line population consisting of 190 lines using single seed descent method.The genetic link-age map was constructed with 202 SSR,CAPs,and STS markers.Quantitative trait loci（QTLs） were identified for eight rice starch RVA profile properties including peak paste viscosity（PKV）,hot paste viscosity（HPV）,cool paste viscosity（CPV）,break-down viscosity（BDV）,setback viscosity（SBV）,consistence viscosity（CSV）,peaktime（PeT）,pasting temperature（PaT） by composite interval mapping method in three different ecological sites（Lingshui,Hefei,and Huaiyuan）.A total of 57 QTLs were identified,with 1 to 14 for each trait,indicating that rice starch RVA profile properties were controlled by multiple genes.Thirteen stable QTLs were detected at two or three sites,among which qCPV-3,qCPV-10b,qSBV-10b,qCSV-3b,and qCSV-10b（explain-ing,26.9%,29.5%,29.7%,25.2%,and 28.3% of variance） were detected in all the three sites.Sixteen QTLs were found to have pleiotropy with a single QTL controlling 2-6 traits（RVA profile properties）.The interval RM25032-RM1375 on chromosome 10 harbored QTLs for six traits（PKV,HPV,CPV,SBV,PaT,and CSV）.

关 键 词：水稻 RVA谱特征值 数量性状 QTL稳定性 QTL多效性 

分 类 号：S511[农业科学—作物学]