低温胁迫下聚糠萘合剂对玉米幼苗光合作用和抗氧化酶活性的影响  

Effects of PASP-KT-NAA on Photosynthesis and Antioxidant Enzyme Activities of Maize Seedlings under Low Temperature Stress

作　　者：徐田军[1] 董志强[1] 兰宏亮[1] 裴志超[1,2] 高娇[1] 解振兴[1] 

机构地区：[1]中国农业科学院作物科学研究所/农业部作物生理生态与栽培重点开放实验室,北京100081 [2]北京市农业技术推广站,北京100029

出　　处：《作物学报》2012年第2期352-359,共8页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(30971726);国家科技支撑计划项目(2006BAD02A13-4)资助

摘　　要：采用盆栽试验,以郑单958和丰单3号为材料,研究了低温胁迫对玉米幼苗光合作用、叶绿素、叶绿素荧光参数和抗氧化酶活性的影响,以及聚糠萘合剂(PKN)的调控效果。结果表明,低温胁迫下,玉米幼苗的光合作用和光系统II光化学最大效率受到抑制;超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性降低;过氧化氢、超氧阴离子的产生速率及丙二醛(MDA)含量显著升高。PKN处理提高了低温胁迫下玉米幼苗净光合速率(Pn)、气孔导度(Gs)、光系统II光化学的最大效率(Fv/Fm)、叶绿素含量(Chla+Chlb)。低温处理7d,郑单958处理(ZDTR)和丰单3号处理(FDTR)的Pn、Gs、Fv/Fm、Chla+Chlb分别比各自的对照提高了88.95%和61.11%、593.33%和1741.67%、111.50%和145.16%、36.61%和54.03%;PKN处理延缓了SOD、POD、CAT活性的降低,低温胁迫7d,PKN处理使郑单958和丰单3号的SOD、POD、CAT活性分别比对照高了292.59%和632.98%、295.07%和360.54%、254.55%和265.45%;同时降低了过氧化氢、超氧阴离子的产生速率及MDA的含量。表明PKN处理有利于提高玉米幼苗的抗冷性。采用盆栽试验,以郑单958和丰单3号为材料,研究了低温胁迫对玉米幼苗光合作用、叶绿素、叶绿素荧光参数和抗氧化酶活性的影响,以及聚糠萘合剂(PKN)的调控效果。结果表明,低温胁迫下,玉米幼苗的光合作用和光系统II光化学最大效率受到抑制;超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性降低;过氧化氢、超氧阴离子的产生速率及丙二醛(MDA)含量显著升高。PKN处理提高了低温胁迫下玉米幼苗净光合速率(Pn)、气孔导度(Gs)、光系统II光化学的最大效率(Fv/Fm)、叶绿素含量(Chla+Chlb)。低温处理7d,郑单958处理(ZDTR)和丰单3号处理(FDTR)的Pn、Gs、Fv/Fm、Chla+Chlb分别比各自的对照提高了88.95%和61.11%、593.33%和1741.67%、111.50%和145.16%、36.61%和54.03%;PKN处理延缓了SOD、A pot experiment of maize seedlings was conducted to investigate the effects of low temperature stress and the chemi-cal regulator of PASP-KT-NAA（PKN） on photosynthetic,physiological parameters,chlorophyll contents,and various antioxidant enzymes activities.The results showed that,in maize seedlings under low temperature stress,the photosynthesis ability and SOD,POD,CAT activities reduced;the formation speed of hydrogen peroxide and superoxide anion,as well as MDA contents in-creased.PKN pretreatment increased Pn,Gs,Fv/Fm,and chlorophyll contents.Under low temperature stress for seven days,Pn,Gs,Fv/Fm,chlorophyll contents of Zhengdan 958 treatment（ZDTR） and Fengdan 3 treatment（FDTR） were higher than those of CK,which was increased by 88.95% and 61.11%,593.33% and 1 741.67%,111.50% and 145.16%,36.61% and 54.03%.While the formation speed of hydrogen peroxide and superoxide anion reduced,MDA contents decreased too.Simultaneously,SOD,POD,CAT activities were increased by 292.59% and 632.98%,295.07% and 360.54%,254.55% and 265.45%.In conclusion,PKN pretreatment can improve the cold resistance of maize seedlings

关 键 词：冷害胁迫 抗氧化酶 光合作用 玉米幼苗 化学调控冷害胁迫 抗氧化酶 光合作用 玉米幼苗 化学调控 

分 类 号：S513[农业科学—作物学]