老年胃癌患者区域转移淋巴结中COX-2与多药耐药因子相关性分析  

Relationship between the expression of cyclooxygenase-2 and multidrug resistance associated factors(P-gp,GST-π,Topo Ⅱα) of gastric carcinomas in elderly

作　　者：安波[1] 檀碧波[1] 李勇[1] 范立侨[1] 赵群[1] 张志栋[1] 赵雪峰[1] 贾楠[1] 王冬[1] 

机构地区：[1]河北医科大学第四医院,河北石家庄050011

出　　处：《中国老年学杂志》2012年第4期692-694,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(No.81072033/H1617);河北省自然科学基金资助项目(No.C2010000619);河北省普通高校强势特色学科资助项目(冀教高〔2005〕52);河北省卫生厅科研基金资助项目(No.20100119)

摘　　要：目的探讨老年患者胃癌原发灶、区域淋巴结转移灶中环氧合酶-2(COX-2)与多药耐药因子P-糖蛋白(P-gp)、谷胱甘肽S转移酶-π(GST-π)及DNA拓扑异构酶Ⅱα(TopoⅡα)表达的关系及意义。方法免疫组化染色法检测33例伴区域淋巴结阳性转移的老年胃癌患者(≥60岁)标本中COX-2、P-gp、GST-π、TopoⅡα的表达情况,同法检测32例对照组(<60岁)胃癌原发灶、转移灶中4种蛋白的表达,比较其在两组患者之间、淋巴结转移灶与原发灶之间的强度表达差异,并进行相关分析。结果老年患者P-gp在转移灶表达高于原发灶(P<0.05),TopoⅡα则在转移灶中表达降低(P<0.05);TopoⅡα表达在原发灶、转移灶间存在正相关关系(r=0.499 3,P<0.01)。老年患者原发灶组织中COX-2与TopoⅡα之间存在负相关表达(r=-0.588 9,P<0.01),P-gp与TopoⅡα之间存在正相关表达(r=0.382 0,P<0.05);转移灶各因子表达间未发现相关关系(均P>0.05)。原发灶老年组COX-2、P-gp和GST-π表达明显高于对照组(均P<0.05);转移灶老年组COX-2、P-gp的表达高于对照组(均P<0.05)。结论老年胃癌患者与对照组比较,COX-2及部分多药耐药因子存在异质性表达,老年患者多药耐药性与其他年龄患者不同,对老年患者进行多药耐药性逆转的胃癌生物治疗应根据COX-2、多药耐药因子的表达特点进行。Objective To investigate the relationship between the expression of cyclooxygenase-2（COX-2） and multidrug resistance associated factors（P-gp,GST-π,TopoⅡα） of gastric carcinomas in elderly.Methods Of the 33 elder patients（≥60 age） and 32 cases in control group,the expressions of COX-2,P-gp,GST-π,TopoⅡαin paired primary tumors（PT） and lymph node metastases（LNMs）of carcinoma specimens were detected by immunohistochemistry.Results The expression of P-gp was significantly higher than that in control group（P〈0.05）,and there was lower expression of TopoⅡα in LNMs（P〈0.05）.Positive correlativity of TopoⅡα was found between PT and LNMs（r=0.499 3,P〈0.01）.In PT of elderly,there was negative correlativity between COX-2 and TopoⅡα（r=-0.588 9,P〈0.01）,and positive correlativity was found between P-gp and TopoⅡα（r=-0.382 0,P〈0.05）.In LNMs of elderly,no correlativity was found among these factors（all P〈0.05）.The expressions of COX-2,P-gp,GST-π in elderly of PT were significantly higher than those in control group（all P〈0.05）;the expressions of COX-2,P-gp were higher in LNMs of elderly than those of control group（both P〈0.05）.Conclusions The heterogenicity exits between elderly and control group in COX-2 and some multidrug resistance associated factors.So molecular target therapy of COX-2 for elder patients with gastric carcinoma should be carried on according to its character.

关 键 词：胃肿瘤 转移 老年人 环氧合酶-2 多药耐药相关因子 

分 类 号：R6[医药卫生—外科学] R753.3[医药卫生—临床医学]