细胞色素2J3基因转染对大鼠胸主动脉球囊损伤后血管狭窄程度的影响及其机制探讨  

Effect and the Mechanism of CYP2J3 Transfection on Rats' Artery Stenosis after Aortic Sac Injury

作　　者：宗哲[1] 付研[1] 李大鹏[1] 程晋[1] 王磊[1] 李建中[1] 常静[2] 张立克[3] 

机构地区：[1]首都医科大学附属北京同仁医院,邮政编码北京100730 [2]首都医科大学附属北京佑安医院 [3]首都医科大学病理生理教研室

出　　处：《微循环学杂志》2012年第1期17-19,22,I0001,共5页Chinese Journal of Microcirculation

基　　金：北京市自然科学基金项目(7062007)

摘　　要：目的:观察细胞色素2J3(CYP2J3)基因转染对实验大鼠胸主动脉球囊损伤后狭窄程度的影响,分析上调CYP2J3/环-二十碳三烯酸(CYP2J3/EETs)系统拮抗血管损伤后狭窄的作用及其机制。方法:建立胸主动脉球囊损伤模型的同时分别经鼠尾静脉注入生理盐水、CYP2J3重组质粒和pcDNA3.1质粒(均为3ml/kg体重),分别为动脉损伤盐水组、动脉损伤CYP2J3组(动脉损伤2J3组)、动脉损伤pcDNA3.1组(动脉损伤3.1组);另取18只大鼠作为假手术组,不进行球囊损伤手术,只经鼠尾静脉注射与手术组相同量的生理盐水(假手术盐水组)、CYP2J3重组质粒(假手术2J3组)和pcDNA3.1质粒(假手术3.1组),每组均为6只大鼠。术后第28天取各组大鼠胸主动脉,分别检测动脉环相对管腔面积(RLA)、CYP2J3mRNA表达水平及11,12-EET含量。结果:动脉损伤各组RLA均小于对应假手术组(P<0.05或P<0.01),动脉损伤2J3组RLA大于动脉损伤盐水组及动脉损伤3.1组(P<0.01)。假手术2J3组CYP2J3mRNA表达高于假手术盐水组及假手术3.1组(P<0.05),动脉损伤各组CYP2J3mRNA表达及11,12-EET水平均高于对应假手术组(P<0.05或P<0.01),动脉损伤2J3组11,12-EET水平高于动脉损伤盐水组。结论:动脉损伤时CYP2J3/EETs系统上调可能是防止狭窄的重要机制。Objective:To investigate the impact of cytochrome 2J3(CYP2J3) gene transfection on experimented rat thoracic aorta stenosis after sac injury,and to analysis the effect and mechanism of up-regulating CYP2J3/epoxyeicosatrienoic acid(CYP2J3/EETs) system rivalried aortic stenosis after sac injury.Method:Establishing the thoracic aorta sac injury model,and meanwhile infusing physiologic saline,CYP2J3 recombinant plasmid and pcDNA3.1 plasmid through rat vena caudalis respectively(equally 3ml/kg),and so were injury physiologic saline group,injury CYP2J3 group and injury pcDNA3.1 group.Another taking 18 rats as sham-operated group,only infusing the same amount of physiologic saline(sham-operated saline group),CYP2J3 recombinant plasmid(sham-operated 2J3 group) and pcDNA3.1 plasmid(sham-operated 3.1 group) through rat vena caudalis,without sac injury.Each group were 6 rats.At the 28 days after aortic sac injury,the relative luminal area(RLA),CYP2J3 mRNA,11,12-EET levels of thoracic aorta were detected.Results:RLA of each injury group were less than the corresponding sham-operated group(P<0.05 or P<0.01),and RLA of injury 2J3 I group were higher than injury saline group and injury 3.1 group(P<0.01).CYP2J3 mRNA expression of sham-operated 2J3 group was higher than sham-operated saline group and sham-operated 3.1 group(P<0.05).CYP2J3 mRNA expression and 11,12-EET content of injury groups was higher than the corresponding sham-operated groups(P<0.05 or P<0.01),and 11,12-EET level of injury 2J3 group was higher than sham-operated 2J3 group and injury saline group.Conclusion:Up-regulating of CYP2J3/EETs system may be an important mechanism for prevention of thoracic aorta stenosis after sac injury.

关 键 词：胸主动脉球囊损伤 血管狭窄程度 基因转染 细胞色素 术后血管再狭窄 血管阻塞性疾病 大鼠 心脑血管疾病 

分 类 号：R543.1[医药卫生—心血管疾病]