糖基化终末产物对大鼠骨髓来源内皮祖细胞增殖、凋亡和管腔形成的影响  

The Influnce of AGEs on Prolifiatation,Apopotosis and Tube Formation of Endothelial Progenitor Cells in Rat Bone Marrow

作　　者：成永霞[1] 刘贵波[2] 孙成[2] 李志强[1] 郭素芬[1] 颜彬[1] 于建渤[1] 杨向红[3] 

机构地区：[1]黑龙江省牡丹江医学院病理教研室,邮政编码牡丹江157011 [2]黑龙江省牡丹江医学院解剖教研室 [3]中国医科大学附属盛京医院病理科

出　　处：《微循环学杂志》2012年第1期30-32,F0004,I0001,共5页Chinese Journal of Microcirculation

基　　金：黑龙江省青年科学基金项目(QC2010040);黑龙江省自然科学基金项目(D2008-83);黑龙江省卫生厅项目(2006-39)

摘　　要：目的:观察糖基化终末产物(AGEs)对骨髓来源内皮祖细胞(EPCs)动员及生物功能的影响。方法:采用不同浓度牛血清白蛋白糖基化终末产物(BSA-AGEs)(50～200mg/L)分别作用EPCs(0～24h),检测EPCs的增殖、凋亡及管腔形成能力的变化。结果:随着AGEs浓度增加及作用时间的延长,EPCs的凋亡率升高,而增殖及管腔形成能力降低(P<0.05)。结论:AGEs对EPCs的存活和增殖具有损害作用,而且还对EPCs功能有破坏作用。Objective:To observe the effect of the advanced glycation end products(AGEs) on prolifiatation,apopotosis and tube formation of endothelial progenitor cells(EPCs).Method:The EPCs were cultured in vitro.After AGEs of the different dose(50～200mg/L)and different times(0～24h) played the role on the cells,the prolifiatation,apopotosis and tube formation of EPCs of all groups were detected.Results:Prolifiatation and tube formation of EPCs decreased with the AGEs dose increasing and treatment time prolong,while EPCs opopotosis rate increased.Statistical analysis of the results have significantly differences.Conclusion:These results demonstrated that AGEs may effect on the vascular recover at diabetets through increase apopotosis of EPCs and decrease its proliferation and tube formation.

关 键 词：内皮祖细胞 糖基化终末产物 细胞增殖 管腔形成 骨髓 大鼠 凋亡 冠状动脉疾病 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]