肿瘤坏死因子-α、白细胞介素-6在高血压心肌肥厚大鼠实验中的意义  

The significance of tumor necrosis factor-α and interleukin-6 in experiment of myocardium hypertrophy

作　　者：史君[1] 王静[2] 王星[1] 孙艳宏[1] 范晓梅[1] 纳仁高娃[1] 

机构地区：[1]内蒙古医学院基础医学院生理教研室,内蒙古呼和浩特010059 [2]内蒙古医学院第一附属医院风湿免疫科,内蒙古呼和浩特010051

出　　处：《中国医药导报》2012年第6期23-25,共3页China Medical Herald

摘　　要：目的探讨肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)在心肌肥厚大鼠血清和心肌中的表达变化及意义。方法 Wistar大鼠20只,随机分成两组,心肌肥厚组(OG)和假手术组(CG),每组10只。腹主动脉部分缩窄法制作压力负荷性心肌肥厚大鼠模型,手术后按要求饲养8周后处死,采血并取心肌标本。采用放射性免疫法检测大鼠血清TNF-α、IL-6含量,应用RT-PCR法检测心肌组织中TNF-α、IL-6 mRNA表达的变化。结果与假手术组比较,心肌肥厚组血清中TNF-α、IL-6含量增加(P<0.05);心肌中TNF-α、IL-6 mRNA表达增多(P<0.05)。结论TNF-α、IL-6参与心肌肥厚的发生和发展过程。Objective To investigate the expression of change and significance about tumor necrosis factor-α（TNF-α）,interleukin-6（IL-6） in serum and myocardial of myocardial hypertrophy rats.Methods 20 Wistar rats were randomly divided into two groups： myocardial hypertrophy group（OG） and control group（CG）.Each group contained 10 rats.The partial abdominal aorta ligature was used to induce hypertrophy in rats.After surgery,rats were feeded according to the requirements for eight weeks,then put to death.Blood and myocardial specimens were obtained.TNF-α and IL-6 content in serum were detected with radioactive immune method.The expression changes of TNF-α mRNA and IL-6 mRNA in myocardial tissue were detected with RT-PCR method.Results Compared with control group,it was found that in myocardial hypertrophic group the content of TNF-α and IL-6 in serum increased greatly（P〈 0.05）;the express of myocardial of TNF-α mRNA and IL-6 mRNA increased（P 〈0.05）.Conclusion TNF-α and IL-6 play a role in myocardial hypertrophy pathogenesis.

关 键 词：肿瘤坏死因子-Α 白介素-6 心肌肥厚 

分 类 号：R542.2[医药卫生—心血管疾病]