用反转录─聚合酶链反应快速鉴别新城疫病毒  

Rapid molecular differentiation of virulent and avirulent strains of Newcastle disease virus with RT PCR technique

作　　者：阎玉河[1] 邓瑞广[1] 赵传璧[2] 刘文惠[1] 李学伍[1] 王爱萍[1] 张玉杨[1] 

机构地区：[1]河南省农业科学院畜牧兽医研究所,河南郑州450002 [2]郑州牧业工程高等专科学校,河南郑州450045

出　　处：《中国兽医科技》2000年第1期3-5,共3页Chinese Journal of Veterinary Science and Technology

摘　　要：根据新城疫病毒基因结构特点及强、弱毒株Ｆ０ 裂解位点的序列差异设计２ 对引物，建立了快速诊断新城疫并能鉴别诊断新城疫强、弱毒株的反转录聚合酶链反应（ ＲＴＰＣＲ） 技术。试验表明，该方法具有快速特异和操作简便等特点，不仅适用于对鸡胚毒的检测，也适用于对病鸡组织匀浆液的检测，是新城疫鉴别诊断和流行病学调查的颇具潜力的分子诊断方法。According to the gene structure of Newcastle disease virus (NDV) and the sequence differences at F0 cleavage sites between virulent and avirulent strains, two primers were designed and the RT PCR method for detecting NDV and differentiating NDV virulent and avirulent strains was developed. The method is rapid, specific and simple for manipulation. It can not only be used for differential diagnosis of NDV strains derived from the inoculated chicken embryo, but also be used for differentiation of the sample of tissue homogenized liquid. It is suggested that this PCR based method is potential for diagnosis of ND, differential diagnosis of NDV and epidemiological investigation.

关 键 词：新城疫病毒 反转录 聚合酶链反应 鉴别 家禽 

分 类 号：S858.305.3[农业科学—临床兽医学] S854.44[农业科学—兽医学]