虫卵孵化试验对某羊场羊线虫抗药性的检测  

Detection of Resistance of Stronglid Nematodeto Albendazole on a Sheep Farm by Egg Hatch Test

作　　者：王权[1] 沈杰[1] 顾建成[2] 

机构地区：[1]中国农业科学院上海家畜寄生虫病研究所,上海200232 [2]江苏省海门市羊场

出　　处：《畜牧与兽医》2000年第1期5-6,共2页Animal Husbandry & Veterinary Medicine

基　　金："九五"国家重点科技攻关项目!(96- 005 - 02 - 01 - 05)

摘　　要：为了解家畜寄生线虫对使用较久的驱虫药丙硫咪唑的抗药性情况， 用虫卵孵化试验对江苏某羊场进行抗药性检测， 测得丙硫咪唑虫卵孵化半数抑制量ＩＤ５０ ＝ ０－０６０ ２ μｇ／ｍＬ， 约是敏感株（０－０２０ ４ μｇ／ｍＬ） 的３ 倍， 但较世界兽医寄生虫促进协会提出的线虫抗药性临界值（０－１ μｇ／ｍＬ） 小。虫卵减少试验也证实了该羊场不存在抗药性。因此该场羊寄生线虫尚无抗药性。In order to understand the resistance of stronglid nematode to albendazole which has been used to animal nematodiasis in China for a long time,egg hatch test(EHT)was used to detect the resistance of haemonchus contortus on a sheep farm in Jiangsu province.The result showed that ID 50 of albendazole was 0 060 2 μg/mL,which was about three times as the susceptive strain,but it was less than 0 1 μg/mL,the lowest resistant value suggested by World Association for the Advancement of Veterinary Parasitology(WAAVP).Faecal egg count reduction test(FECRT)also confirmed that there was no resistance to albendazole on this farm.

关 键 词：虫卵孵化试验 抗药性 驱虫药 羊 线虫 

分 类 号：S859.795[农业科学—临床兽医学] S858.265.9[农业科学—兽医学]