晚孕期孕妇口服益生菌制剂对新生儿肠道菌群及脐血IL-4、IFN-γ水平影响的临床研究  

Clinical research of the effect of oral administration of probiotic products among the pregnant women during the third trimester of pregnancy on intestinal flora and levels of IL-4,IFN-γ in umbilical cord blood of the neonates

作　　者：刘明颖[1,2] 李辉[1,2] 施展 金良怡[1,2] 

机构地区：[1]中国医科大学附属盛京医院妇产科,辽宁沈阳110004 [2]大连妇产医院

出　　处：《中国妇幼保健》2012年第6期923-925,共3页Maternal and Child Health Care of China

摘　　要：目的:通过给妊娠晚期孕妇服用含有益生菌的孕妇奶粉,检测其新生儿肠道菌群变化及新生儿脐血中IL-4、IFN-γ的变化,探讨益生菌对新生儿免疫的影响。方法:随机将妊娠34周择期剖宫产的孕妇分为实验组(服用含益生菌的孕妇奶粉21例)及对照组(服用不含益生菌的孕妇奶粉21例),取新生儿粪便分别进行有氧及厌氧环境培养,并进行细菌涂片,观察新生儿肠道菌群变化情况。剖宫产收集新生儿脐带血液标本,留取血清,采用ELISA方法测定两组中IL-4及IFN-γ水平。结果:①实验组与对照组新生儿肠道菌群变化两组间无统计学差异(P>0.05);②实验组与对照组新生儿脐带血清IL-4水平两组之间有统计学差异(P<0.05),IFN-γ水平两组之间无明显统计学差异(P>0.05),而IL-4/IFN-γ比值两组之间有统计学差异(P<0.05)。结论:晚孕期孕妇口服益生菌制剂对新生儿肠道菌群变化无明显影响,但通过新生儿脐带血IL-4及IFN-γ的检测发现晚孕期孕妇口服益生菌制剂可能会增强新生儿免疫力,从而预防或减少新生儿过敏性疾病的发生。Objective：To detect the changes of intestinal flora and levels of IL-4,IFN-γ in umbilical cord blood of the neonates by oral administration of probiotic products among the pregnant women during the third trimester of pregnancy,explore the effect of probiotics on neonatal immunity.Methods：Thirty-four pregnant women who adopted elective cesarean section were divided into experimental group and control group randomly,21 pregnant women in experimental group were treated with milk powder containing probiotics,while 21 pregnant women in control group were treated with milk powder without probiotics,the neonatal feces were obtained for bacterial culture under aerobic environment and anaerobic environment,respectively,then bacterial smears were performed to observe the changes of intestinal flora of neonates.After cesarean section,the umbilical cord blood samples of neonates were collected,then serum samples were obtained,ELISA was used to detect the levels of IL-4 and IFN-γ in the two groups.Results：There was no significant difference in the changes of intestinal flora of neonates between the two groups（P0.05）;there was significant difference in umbilical cord serum level of IL-4 between the two groups（P0.05）;but there was no significant difference in umbilical cord serum level of IFN-γ（P0.05）;there was significant difference in the ratio of IL-4/IFN-γ between the two groups（P0.05）.Conclusion：Oral administration of probiotic products among the pregnant women during the third trimester of pregnancy has no obvious impact on the changes of intestinal flora of neonates,but after IL-4 and IFN-γ detection in umbilical cord of neonates,it is indicated that oral administration of probiotic products among the pregnant women during the third trimester of pregnancy may enhance neonatal immunity,and prevent or reduce the occurrence of neonatal allergic diseases.

关 键 词：益生菌 IL-4 IFN-Γ 脐血 新生儿粪便 

分 类 号：R725.7[医药卫生—儿科]