蜥蜴利什曼原虫在小鼠体内的分布及增殖研究  

Distribution and Proliferation of Leishmania tarentolae in BALB/c Mice

作　　者：李琳[1,2] 马丽敏[1,2] 任秀梅[1] 何叶[1] 李晔[1] 商利民[2] 胡乐鹏[2] 薛慧亮[2] 李丹[2] 靳朝[1] 夏志平[2] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]中国人民解放军军事医学科学院军事兽医研究所,吉林长春130122

出　　处：《中国畜牧兽医》2012年第2期48-51,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家高技术研究发展计划(2009AA10Z4483730)

摘　　要：通过腹腔途径用体内含有荧光蛋白的蜥蜴利什曼原虫感染BALB/c小鼠,感染后采其脏器做冰冻切片,荧光染料染色后用荧光显微镜观察,并经PCR鉴定,结果显示蜥蜴利什曼原虫感染小鼠后,主要分布于心脏、肝脏、脾脏、肺脏、肾脏。另外,成功建立了利什曼原虫荧光定量PCR检测方法,并采用该方法检测感染蜥蜴利什曼原虫后BALB/c小鼠体内利什曼原虫的增殖情况,结果显示,感染13d内,BALB/c小鼠体内蜥蜴利什曼原虫呈波浪状增殖。这一结果为研究蜥蜴利什曼原虫感染人和动物的致病机理和免疫方法及疫苗研制等方面提供了基础理论依据。BALB/c mice inoculated with Leishmania tarentolae parasites which express fluorescent protein by intraperitoneal routes.Frozen sections of their organs were stained with fluorescent dye and observed by fluorescence microscopy.The results showed that Leishmania tarentolae were observed in heart,liver,spleen,lung and kidney of BALB/c mice.In addition,Leishmania real-time quantitative PCR method was established successfully and used to detect Leishmania tarentolae in BALB/c mice.These results indicated that the proliferation curve of Leishmania tarentolae in BALB/c mice was wavy in 13 days.The results for the study of Leishmania tarentolae will provide the theoretical basis for pathogenesis,vaccine development in humans and animals.

关 键 词：蜥蜴利什曼原虫 荧光染色 实时荧光定量PCR 

分 类 号：S879.1[农业科学—畜牧兽医]