取代基对黄酮类化合物流感病毒神经氨酸酶抑制活性的影响  

Influence of Substituent on the Neuraminidase Inhibitory Effect of Flavonoids

作　　者：胡凤杨[1] 陆春妮[1] 汤有志[1,2] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]广东省兽药研制与安全评价重点实验室,广东广州510642

出　　处：《中国畜牧兽医》2012年第2期144-147,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(31001085);教育部博士点新教师基金(20104404120013);华南农业大学大学生科技创新活动项目(L10088)

摘　　要：本试验旨在研究取代基对黄酮类化合物流感病毒神经氨酸酶(neuraminidase,NA)抑制活性的影响,为黄酮类流感病毒神经氨酸酶抑制剂的研发提供理论依据。应用流感病毒神经氨酸酶活性抑制试验评价了4种黄酮类化合物对NA的抑制活性。芹菜素-7-葡萄糖苷、芹菜素具有H9N2亚型流感病毒NA抑制活性,蒙花苷、汉黄芩苷无H9N2亚型流感病毒NA抑制活性。4’位的取代羟基对于黄酮类化合物的NA抑制活性不可或缺,7位的糖苷键能增强该类化合物的NA抑制活性。The substituent effects on neuraminidase（NA） inhibitory effect of flavonoids have been studied and could be used to provide experimental data for the exploitation of flavonoids as NA inhibitors.An assay method for evaluating the inhibitory activity of compounds on influenza viral NA was established and was used to evaluate the inhibitory effects of four flavone derivatives.Apigenin-7-glucoside and apigenin possessed inhibitory activity on H9N2 influenza viral NAs.Linarin and wogonoside did not possess inhibitory activity on H9N2 influenza viral NAs.The 4＇-OH were essential for NA inhibitory effects of flavonoids and the 7-glycosidic bond could increase NA inhibition of flavonoids.

关 键 词：黄酮 芹菜素-7-葡萄糖苷 芹菜素 神经氨酸酶 抑制活性 

分 类 号：S859.7[农业科学—临床兽医学]