氯胺酮对大鼠蓝斑核和内侧前额叶皮质c-Fos蛋白表达的影响  

EFFECTS OF KETAMINE ON C-FOS PROTEIN EXPRESSION IN LOCUS COERULEUS AND MEDIALPREFRONTALCORTEX IN RATS

作　　者：李刚[1] 纪蒙蒙[1] 刘慧[1] 高磊磊[1] 崔东红[2] 于剑锋[1] 

机构地区：[1]潍坊医学院麻醉学系,潍坊261053 [2]上海市精神卫生中心,上海200030

出　　处：《中国疼痛医学杂志》2012年第2期99-103,共5页Chinese Journal of Pain Medicine

基　　金：潍坊医学院大学生科技创新基金(KX2009012)资助课题

摘　　要：目的:检测氯胺酮对大鼠蓝斑核(locus coeruleus,LC)和内侧前额叶皮质(medial prefrontalcortex,mPFC)c-Fos蛋白表达的影响。方法:24只Wistar大鼠随机分为4组(n=6):对照1组、2组(C1组、C2组),氯胺酮1组、2组(K1组、K2组),分别腹腔注射生理盐水,氯胺酮100 mg/kg。C1组和K1组用于检测大鼠用药120 min内的行为学变化和第120 min时的热痛阈值;C2组和K2组用于通过Western blotting方法检测用药第120 min时,LC和mPFC脑区Fos蛋白的表达水平。结果:K1组较C1组热痛阈值明显升高(P<0.05),运动亢进、刻板行为也有显著差异(P<0.01)。K2组较C2组LC和mPFC处Fos蛋白表达均明显增多(P<0.05和P<0.01)。结论:氯胺酮可引起热痛镇痛效应和行为异常,伴有LC和mPFC的Fos蛋白表达增加。Objective：To detect the effects of ketamine on c-Fos protein expression in locus coeruleus（LC） and medial prefrontal cortex（mPFC） in the rats.Methods： 24 Wistar rats were randomly divided into 4 groups（n = 6）： control 1,2 group（C1,C2 group） with intraperitoneal injection of normal saline;ketamine-treated 1,2 group（K1,K2 group） with intraperitoneal injection of ketamine 100 mg/kg.C1 and K1 groups were used to detect the behavior changes within 120 min and thermal pain thresholds at 120 min;C2 and K2 groups were used to detect c-Fos protein expression level in LC and mPFC tissues at 120 min by Western blotting.Results： Thermal pain thresholds in K1 group was significantly higher（P 0.05） than in C1 group accompanying with hyperactivity and stereotyped behavior（P 0.01）.The c-Fos protein expression in LC and mPFC of rats increased significantly in K2 group compared with that in C2 group（P 0.05,P 0.01,respectively）.Conclusion： Ketamine may induce analgesic effects on thermal pain and behavior disorder accompanied with increased c-Fos protein expression in LC and mPFC.

关 键 词：氯胺酮 蓝斑核 内侧前额叶皮质 FOS蛋白 大鼠 

分 类 号：R965[医药卫生—药理学]