脐血内皮祖细胞中整合素连接激酶在子痫前期发病机制中的作用  

Role of integrin linked kinase expression in endothelial progenitor cells from cord blood of preeclampsia patients in the pathogenesis of preeclampsia

作　　者：王芳[1] 邹丽[1] 王莹[1] 张莉[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院妇产科,武汉430022

出　　处：《中华围产医学杂志》2012年第2期101-105,共5页Chinese Journal of Perinatal Medicine

摘　　要：目的探讨整合素连接激酶（integrinlinkedkinase，ILK）在子痫前期患者脐血内皮祖细胞中的表达及其在新生血管形成中的作用。方法采用逆转录聚合酶链反应和Western印迹实验分别检测35例正常孕妇（正常妊娠组）和30例子痫前期孕妇（子痫前期组，其中轻度18例，重度12例）的脐血内皮祖细胞中ILKmRNA和蛋白的表达，采用小管形成实验检测内皮祖细胞血管形成能力；分析ILKmRNA和蛋白表达的差异及其与血管管状结构形成的相关性。统计学分析采用方差分析和相关分析。结果（1）正常妊娠组内皮祖细胞中ILKmRNA相对吸光度（A）值较子痫前期组显著升高（O．64±0．05与0．45±0．06），轻度子痫前期组较重度者显著升高（0．47±0．07与0．39±0．08）（q=18．76和5．13，P〈0．05）；正常妊娠组内皮祖细胞中ILK蛋白A值较子痫前期组明显升高（32±2与26±1），轻度子痫前期组较重度者显著升高（25±2与20±2）（q：18．47和4．72，P均〈O．05）。（2）正常妊娠组小管结构形成的数量较子痫前期组显著增多（330±8与135±7），重度子痫前期组的小管形成的数量明显少于轻度者（116±8与148±6）（q=152．70和5．42，P均〈0．05）。（3）内皮祖细胞中ILKmRNA及其蛋白表达与其小管形成均呈正相关关系，正常妊娠组相关系数（r）分别为0．69和0．73，子痫前期组分别为0．67和0．72；轻度子痫前期组分别为0．65和0．68，重度组分别为0．63和0．74（P均〈0．05）。结论子痫前期患者内皮祖细胞中ILKmRNA及其蛋白表达下降可能与子痫前期的发生密切相关。Objective To investigate the expression of integrin linked kinase （ILK） in endothelial progenitor cells （EPC） of cord blood from patients with preeclampsia and its relation to placenta perfusion. Methods Thirty patients with preeclampsia, including 18 mild preeclampsia and 12 severe preeclampsia cases, and thirty-five normal late pregnant women were enrolled into this study. The expression of ILK mRNA and protein in EPC of cord blood was determined with RT-PCR and Western blot respectively. The angiogenesis ability of EPC was examined by tube formation in vitro. Comparisons of ILK mRNA and protein expression were done by analysis of variance and their relations to vascular tube formation were analyzed by correlation analysis. Results （1） ILK mRNA expression in normal group was higher than that in preeclampsia group （0.64±0.05 vs 0.45 ± 0.06, q=18.76,P〈0.05） and the ILK protein expression in normal group was also higher （32±2 vs 26±1, q= 18.47, P〈0.05）. While the ILK mRNA and protein expression in mild preeclampsia group were also higher than that of severe preeclampsia group （ILK mRNA 0. 47±0.07 vs 0. 39± 0.08,q=5.13,P〈0.05;ILK protein： 25±2 vs 20±2,q=4.72,P〈0.05）. （2） The tube formation capacity in preeclampsia group was significantly lower than that in normal group （135 ± 7 vs 330 5= 8, q：152.70,P〈0. 05） ; the capacity was even much lower in severe group than in mild group （116=t=8 vs 148±6, q=5.42,P〈0.05）. （3） There was positive correlation between ILK mRNA and protein expression and tube formation capacity; the correlation index for normal group was 0.69 and 0.73 （P〈0. 05）, 0.67 and 0.72 in preeclampsia group （P〈0.05）, 0. 65 and 0. 68 in mild preeclampsia group （P〈0.05） and 0.63 and 0.74 in severe preeclampsia group （P〈0.05）. Conclusions Decreased ILK level of EPC in cord blood might play an important role in the development of preeclampsia.

关 键 词：先兆子痫 胎血 内皮 血管 干细胞 蛋白质丝氨酸苏氨酸激酶 

分 类 号：R714.245[医药卫生—妇产科学]