产前应用地塞米松和沐舒坦对胎鼠及仔鼠肺组织Toll样受体4表达影响的比较  

Comparison of effects of prenatal administration of dexamethasone and ambroxol on expression of Toll- like receptor 4 of fetal and neonatal rats

作　　者：浦潇丹[1] 阚清[1] 陈筱青 吴越[1] 缪晓林 周晓玉[1] 

机构地区：[1]南京医科大学附属南京儿童医院新生儿科,210008 [2]南京医科大学第一附属医院河西分院儿科

出　　处：《中华围产医学杂志》2012年第2期113-118,共6页Chinese Journal of Perinatal Medicine

摘　　要：目的比较产前单次应用小剂量地塞米松与联合应用地塞米松加盐酸氨溴索（沐舒坦）对胎鼠及仔鼠肺Toll样受体4（Toll—likereceptor4，TLR4）基因及蛋白表达的影响。方法54只SD孕鼠随机分为3组：地塞米松组（干预组1）、地塞米松加沐舒坦组（干预组2）和生理盐水对照组。妊娠17d，腹腔注射给药，干预组1予地塞米松0．2mg／kg，干预组2予地塞米松0．2mg／kg加沐舒坦100mg／kg，对照组予等体积生理盐水。分别在妊娠19d及生后3、7d取胎（仔）鼠肺组织，通过逆转录聚合酶链反应、免疫组织化学和蛋白质免疫印迹技术检测各组胎（仔）肺组织中TLR4基因转录水平及蛋白表达差异。统计学方法采用方差分析和独立样本t检验。结果（1）孕19d，2个干预组分别与对照组相比，TLR4mRNA在胎鼠肺组织中的表达均明显增加（对照组：0．264-_0．18，干预组1：0．39±0．21，t=5．866，P〈0．05；对照组：0．27±0．22，干预组2：0．46±0．13，t=9．572，P〈0．01）。生后3d和7d干预组2与对照组相比，TLR4inRNA在仔鼠肺组织中的表达均明显增加（生后3d：0．59±0．23与0．47±0．24，t=2．295，P〈0．05；生后7d：0．52±0．12与0．35±0．17，t=4．219，P〈0．05），但干预组1与对照组比较，差异无统计学意义（生后3d：0．45±0．22与0．44±0．14，t=0．128，P〉0．05；生后7d：0．40±0．16与0．36±0．12，t=1．365，P〉O．05）。（2）免疫组织化学：孕19d，2个干预组分别与对照组相比，TLR4蛋白表达量在胎鼠肺组织中的表达均明显增加（对照组：0．20±0．29，干预组1：0．35±0．32，t=7．179，P〈0．05；对照组：0．20±0．29，干预组2：0．39±0．25，t=10．764，P〈O．01）。生后3d和7d干预组2与对照组相比，TLR4蛋白表达量在仔鼠肺组织中的表达均明显增加（生后3d：0．55±0．32与0．37±0．18，t=7．121，P〈O．05；�Objective To investigate the role of prenatal single-dose administration of dexamethasone and ambroxol on the expression of Toll-like receptor 4 （TLR4） of fetal and neonatal rats. Methods Fifty-four pregnant rats were randomly divided into three groups with eighteen rats in each group： rats treated with 0.2 mg/kg dexamethasone （group 1）, 0.2 mg/kg dexamethasone and 100 mg/kg arnbroxol （group 2）, or saline（controls） on the 17th day of gestation. The lung tissues of the offsprings were harvest independently on the 19th day of gestation, the postnatal 3 days and 7 days. The expressions of TLR4 in fetal/neonatal rat lungs of each pregnant rat were analyzed by reverse transcription-polymerase chain reaction（RT-PCR）, immunohistochemistry stain, and Western blot. ANOVA and two independent samples t-test were applied. Results On the 19th day of pregnancy, TLR4 mRNA expression was up-regulated in lungs of the two treatment groups compared with controls （controls .. 0.26± 0.18, group 1 ： 0.39± 0.21, t = 5.866, P 〈 0.05 ; control： 0.27±0.22, group 2 ： 0.46 ± 0.13, t = 9. 572, P 〈0.01）. TLR4 mRNA expression was up-regulated in group 2 compared with controls on the postnatal 3 days and 7 days （postnatal 3 d： 0. 59 =k 0. 23 and 0. 47 ± 0.24,t=2. 295,P〈0.05 postnatal 7 d：0.52±0.12 and 0. 35±0.17,t=4. 219,P〉0.05）, while no significant difference was found in group 1 compared with the controls（postnatal 3 d..0. 45±0.22 and 0. 44±0.14,t= 0. 128,P〈0.05;postnatal 7 d..0. 40±0.16 and 0. 36± 0.12,t= 1. 365,P〉0.05）. Results of the immunohistochemistry demonstrated that on the 19th day of pregnancy, the protein expression of TLR4 was significantly increased in the two treatment groups （controls： 0. 20 ± 0.29, group 1..0. 35±0.32,t=7. 179,P〈0.05controls：0. 20±0.29,group 2：0. 39±0.25,t=10. 764,P〈 0.01）. The protein expression of TLR4 was significantly increased in group 2 on the postnatal 3 days and 7 days（postnatal 3 d：0. 55±0.32 and 0. 37±0.18,t=7

关 键 词：地塞米松 氨溴索 肺 TOLL样受体4 胎儿器官成熟度 大鼠 

分 类 号：R722.1[医药卫生—儿科]