^(18)F-FDG对小鼠Lewis肺癌移植瘤抑制作用的实验研究  

Effects of ^(18)F-FDG on the growth of Lewis lung cancer xenografts in C57BL/6 mice

作　　者：吴翠萍[1] 邱俊[1] 蔡二朋[1] 朱梅[1] 宋烈晶[1] 王明明[1] 

机构地区：[1]安徽医科大学放射医学研究所,合肥230032

出　　处：《辐射研究与辐射工艺学报》2012年第1期27-31,共5页Journal of Radiation Research and Radiation Processing

基　　金：安徽省教育厅自然科学基金(KJ2011Z162);中国科学院新型薄膜太阳电池重点实验室开放基金(2010007)资助

摘　　要：建立Lewis肺癌C57BL/6小鼠皮下移植瘤模型18只,按随机数字法分为高剂量18F-FDG治疗组、低剂量18F-FDG治疗组和对照组,每组6只,分别给予18F-FDG 18.5×107Bq、9.25×107Bq和0.2 mL等体积的生理盐水,于接种后第7天腹腔内一次给药,观察18F-FDG对Lewis肺癌移植瘤体积变化和瘤重的影响。22天后用流式细胞仪检测细胞凋亡,以免疫组化法检测bcl-2及Survivin基因的蛋白表达。发现18F-FDG高、低剂量组肿瘤抑瘤率及凋亡率明显高于对照组,Bcl-2及Survivin蛋白的表达明显低于对照组(P<0.05),与低剂量组相比,高剂量组与对照间差异更为显著(P<0.01)。这表明18F-FDG可明显抑制小鼠Lewis肺癌移植瘤生长,其机制可能与下调Bcl-2及Survivin蛋白的表达促进细胞凋亡有关。Model of Lewis lung cancer xenograft in C57BL /6 mouse were set up with 18 mice,which were randomly divided into three groups： high-dose 18F-FDG group,low-dose 18F-FDG group and control group.The control was given 0.2 mL saline by intraperitoneal injection 18.5×107Bq and 9.25×107 Bq 18F-FDG in 0.2 mL saline was given intraperitoneally for one time on the 7th day after Lewis lung cancer cells were inoculated in the high dose 18F-FDG group and low-dose 18F-FDG group,respectively.The effects of 18F-FDG on animals and xenografts were investigated.After 22 days,expression of Bcl-2 and survivin proteins was detected using immunohistochemistry,and apoptotic rate were determined by flow cytometry.The results showed that the inhibition rate and apoptotic rate of xenografts in 18F-FDG-treated groups were higher than that in control group,and the expression of Bcl-2 and Survivin proteins in 18F-FDG-treated groups were less than that in control group（P〈0.05）.Compared with low-dose 18F-FDG group,there were more significant differences between the high-dose 18F-FDG group and control（P〈0.01）.It can be suggested that18F-FDG could effectively inhibit the growth of xenografts.The mechanism may be related to apoptosis caused by the down regulation of bcl-2 and survivin gene expressions in xenografts.

关 键 词：18氟-氟代脱氧葡萄糖 LEWIS肺癌 细胞凋亡 BCL-2基因 SURVIVIN基因 

分 类 号：R817.5[医药卫生—影像医学与核医学]