塞来昔布对大鼠急性放射性脑损伤的保护作用  

Neuroprotective effect of Celecoxib on radiation-induced acute brain injury

作　　者：杨美玉[1] 徐晓婷[1] 涂彧[1] 马辰莺[1] 

机构地区：[1]苏州大学医学部放射医学与防护学院江苏省放射医学与防护重点实验室,苏州215123

出　　处：《辐射研究与辐射工艺学报》2012年第1期32-36,共5页Journal of Radiation Research and Radiation Processing

基　　金：江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：为探讨塞来昔布对电离辐射诱发的急性脑组织损伤的脑保护作用,将成熟的SD大鼠随机分为对照组、照射组(6 MeV电子线全脑单次垂直照射,吸收剂量为10 Gy)和照射加药物组(6 MeV电子线全脑单次垂直照射,吸收剂量为10 Gy+塞来昔布30 mg/kg体重灌胃给药)。实验大鼠分别在不同时间点处死、取脑组织。应用干-湿重法测定脑组织含水量,病理切片观察大鼠脑组织形态变化,免疫组化法观察脑组织中胶质纤维酸性蛋白(GFAP)的表达。结果显示:与对照组相比,SD大鼠照射后脑组织含水量明显升高,照射加药物组大鼠各时间点脑组织含水量较照射组显著减少,差异有统计学意义(P<0.05)。全脑照射后,照射组大鼠脑组织切片呈现典型脑水肿表现,照射加药物组脑水肿程度较照射组轻。照射加药物组大鼠各时间点脑组织中GFAP阳性细胞的数量较照射组显著减少,差异有统计学意义(P<0.05),两组全脑照射后大鼠脑组织中GFAP阳性表达均较对照组明显升高。塞来昔布能有效地降低脑组织含水量,减轻脑组织水肿的程度,保护脑组织,并能减轻胶质细胞的损伤程度,起到保护神经元的作用。In order to explore the effect of Celecoxib on the neuroprotective function of acute radiation-induced brain injury,the mature Sprague-Dawley rats were divided randomly into 3 groups： the control group,the 10 Gy group（a dose of 10 Gy by using 6 MeV elections） and the 10 Gy＋Celecoxib group（a dose of 10 Gy by using 6 MeV elections combined with 30 mg/kg of Celecoxib）.The brain tissues of rats were taken at the different points of time after irradiation and the wet-dry weight formula was applied to calculate the percentage of brain water content.Pathomorphology investigation was used to detect the morphological changes of brains.Immunohistochemistry staining was performed on the brain tissue samples to investigate the glial fibrillary acidic protein（GFAP） expression.Consequently,the percentage of brain water content in the 10 Gy group was significantly higher than that in the control group.And the percentage of brain water content in the 10 Gy＋Celecoxib group was relatively lower compared with that in the 10 Gy group.There were significant differences among the three groups（P〈0.05 and P〈0.05）.Pathologic identification of the brain tissue samples suggests that typical cerebral edema was detected in the 10 Gy group,and the extent of brain edema was less in the 10 Gy＋Celecoxib group compared with that in the 10 Gy group,meanwhile the positive expression of GFAP in the 10 Gy＋Celecoxib group was lower significantly than that in the 10 Gy group at the different time points（P〈0.05）.The positive expression of GFAP in both the 10 Gy group and the 10 Gy＋ Celecoxib group were higher than that in the control group.As a conclusion,Celecoxib can obviously decrease the percentage of brain water content,cut down the level of cerebral edema to protect brain organization,reduce the glial cells injury and protect neurons.

关 键 词：塞来昔布 放射性脑损伤 大鼠 脑水肿 6 MeV电子线 

分 类 号：R811.5[医药卫生—放射医学] R815.6[医药卫生—临床医学]