干酪乳杆菌JH-23中单胺氧化酶抑制剂的初步分离  

Separation of Monoamine Oxidase Inhibitor Originated from Lactobacillus casei JH-23

作　　者：王豪[1] 周凌华[1] 吴正钧[1] 王荫榆[1] 何楚莹[1] 陈卫[2] 

机构地区：[1]光明乳业股份有限公司技术中心乳业生物技术国家重点实验室,上海200436 [2]江南大学食品学院食品科学与技术国家重点实验室,无锡214122

出　　处：《天然产物研究与开发》2012年第2期208-211,共4页Natural Product Research and Development

基　　金：国家"十一五"科技支撑计划项目(2006BAD04A14);上海市农业成果转化项目(083919n1500)

摘　　要：采用丙酮抽提法得到了干酪乳杆菌JH-23中具有抑制单胺氧化酶作用的无细胞提取物,并利用硅胶薄层色谱从中分离出4组活性片段,其中片段Ⅰ对单胺氧化酶的抑制效果最佳,反应浓度在120μg/mL时抑制率为55.4%。该活性片段经国家新药筛选中心确证有抑制单胺氧化酶的效果,具有潜在的抗衰老作用。Cell-free extracts derived from Lactobacillus casei JH-23,with monoamine oxidase inhibition,were obtained by acetone extraction method.Moreover,four active fractions from extracts were separated by silica thin layer chromatogram,among which the fraction I displayed the strongest monoamine oxidase inhibitory activity,with an inhibitory rate reached 55.4% at concentration of 120 μg/mL.National Center for Drug Screening confirmed that the bioactive fraction was reliably inhibitory to monoamine oxidase and possessed potential anti-ageing effect.

关 键 词：干酪乳杆菌JH-23 单胺氧化酶 丙酮抽提 薄层色谱 

分 类 号：R284.2[医药卫生—中药学]