Cre-LoxP系统调控肠道粘蛋白2启动子活性和特异性  

Cre-LoxP System Regulates the Activity and Specificity of Mucin 2 Promoter

作　　者：翟亚峰[1] 束刚[1] 张志岐[1] 朱晓彤[1] 江青艳[1] 李加琪[1] 吴珍芳[1] 

机构地区：[1]华南农业大学动物科学学院,广州510642

出　　处：《农业生物技术学报》2012年第2期152-156,共5页Journal of Agricultural Biotechnology

基　　金：国家转基因生物新品种培育科技重大专项(No.2008ZX08006-004)

摘　　要：靶基因的高丰度组织特异性表达是基因治疗和制备转基因动物的重要条件。运用Cre-LoxP系统是提高组织特异性启动子转录活性的有效途径之一。本研究利用Cre-LoxP系统,以肠道粘蛋白2启动子调控Cre重组酶表达,转染细胞后检测荧光素酶活性。结果显示,Cre-LoxP系统能使粘蛋白2启动子介导的靶基因在肠细胞SW480中的表达量提高约6倍。细胞特异性检验后发现,Cre-LoxP系统显著弱化了肠道粘蛋白2启动子的细胞特异性。研究结果提示,运用Cre-LoxP系统尽管可以大幅提高弱启动子的活性,但对启动子的特异性要求较高。High abundance and tissue-specific gene expression are important for gene therapy and preparation of transgenic animals.Cre-LoxP system is effective to improve transcriptional activity of tissue-specific promoter.However,it is not known if the Cre-LoxP system affects the tissue-specific expression regulated by the specific promoter..In this study,intestinal mucin 2 promoter was used to regulate the Cre recombinase expression base on Cre-LoxP system.Then luciferase activity was detected after transfection.The results showed that Cre-LoxP system increased the expression of mucin 2 promoter-mediated target genes（6-fold） in intestinal cells.However,cell-specific tests showed that the system decreased the specificity of mucin 2 promoter.The results suggest that the use of Cre-LoxP system requires higher specificity of the promoter although the system can significantly improve the weak promoter activity.

关 键 词：Cre-LoxP系统 肠道粘蛋白2启动子 转基因 基因治疗 

分 类 号：S942.5[农业科学—水产养殖]