猕猴自然流产胎儿胸腺白细胞介素2和10的分布与表达  

Distribution and Expression of Interleukin-2 and-10 in Thymus of Spontaneous Aborted Foetus of Rhesus Macaques(Macacca mulatta)

作　　者：贾姣[1] 李冰[1] 赖茂银[1] 杨丽莎[1] 陈正礼[1,2] 程安春[1,2,3] 曾文[2] 

机构地区：[1]四川农业大学动物疫病与人类健康四川省重点实验室,雅安625014 [2]四川农业大学实验动物工程技术中心国家实验用猕猴种源基地,雅安625014 [3]四川农业大学预防兽医研究所,成都611130

出　　处：《农业生物技术学报》2012年第2期157-164,共8页Journal of Agricultural Biotechnology

基　　金：教育部"长江学者和创新团队发展计划"创新团队项目(No.IRT0848);四川省国际科技合作与交流研究计划项目(No.2010HH0013);四川省科技厅应用基础项目(No.2011JY0054);四川省博士后引进项目专项(No.04310608)

摘　　要：胸腺是哺乳动物的中枢免疫器官。为了解猕猴胎儿中枢免疫器官在自然流产时的免疫应答情况,采用猕猴(Macaca mulatta)自然流产胎儿为材料,通过HE染色和免疫组化染色对不同胎龄猕猴胎儿胸腺白细胞介素2和10(IL-2;IL-10)阳性细胞的分布及表达进行观测,研究其在自然流产过程中的表达情况。结果表明:猕猴胎儿胸腺各胎龄组小叶间分界清晰,小叶数量皮质髓质中淋巴细胞数量也逐月增加;2月胚龄髓质形成,开始出现弥散型胸腺小体,3月胚龄髓质中可见典型胸腺小体,胸腺组织结构趋于完善。胸腺IL-2和IL-10的表达在1月龄无阳性,此后阳性细胞的光密度值和阳性细胞面积率随着月龄的增加而增长;阳性细胞分布于皮质边缘、皮质与髓质交界处、髓质中心区,以及没有退化的胸腺小体和血管周围;IL-2在胸腺中的表达强烈且数量多,IL-10表达较少。研究结果提示两种细胞因子主要在胸腺细胞发育增长活跃区域表达,并以IL-2表达为主,IL-2和IL-10的分泌水平可作为预测孕妇早产的一个新的敏感指标。Mammalian thymus is the central immune organ.In order to investigate the immune response of the central immune organ in fetal rhesus macaques when aborted,spontaneous aborted fetuses were used as experimental material and the distribution and expression of their interleukin-2 and-10（IL-2＋ and IL-10＋） cells were determined by HE staining and immunohistochemical staining in different gestational age of fetal rhesus macaques.Results：Interlobular dividing lines of the fetal thymus in macaques in each gestational age group were clear.The number of lobules and lymphocytes in cortical medulla were increased by month-to-month.In two-month embryo age,medulla was formatted and diffuse thymus vorkommen was appeared.,In three-month embryo age,typical thymus vorkommen was observed in medulla and the thymus structure was organized.Thymus IL-2 and IL-10 expression in the 1-month-old was negative.After that,the optical density and the area ratio of positive cells were increased with the development of age.Positive cells were distributed in the cortical edge,the junction of cortex and medulla,the central area of the medulla,and the thymus body without degradation and blood vessels around the thymus.Furthermore,the expression of IL-2 was high,whereas the expression of IL-10 was low.The results suggest that two cytokines,especially IL-2,were mainly expressed in thymocytes＇ growth area.In addition,secretion levels of IL-2 and IL-10 can be as a predictor of premature of pregnant women.

关 键 词：自然流产 胸腺 白细胞介素2(IL-2) 白细胞介素10(IL-10) 免疫组织化学 

分 类 号：S858.235.7[农业科学—临床兽医学]