Simultaneous Removal of Thiophene and Dibenzothiophene by Immobilized Pseudomonas delafieldii R-8 cells  

固定化Pseudomonas delafieldii R-8细胞同时脱除噻吩和二苯并噻吩(英文)

作　　者：唐煌 李强 王泽龙 闫道江 邢建民 

机构地区：[1]National Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China [2]Graduate University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Chinese Journal of Chemical Engineering》2012年第1期47-51,共5页中国化学工程学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China (21076216,30970046)

摘　　要：Biodesulfurization （BDS） is a promising technology for deep desulfurization. In this work, Pseudomonas delafieldii R-8 cells are immobilized in calcium alginate beads and used for BDS of transportation fuels. It is found that thiophene and dibenzothiophene （DBT） can be simultaneously metabolized by immobilized R-8 cells. The initial sulfur content in the model oil is 300 mg·kg-1 （thiophene ＂ DI3T= 1 ＂ 1）. After 10 h of treatment, the thiophene concentration is reduced by 40%, while DBT is reduced by 25%. The utilization rate of thiophene is faster than that of DBT. Moreover, the oil/water ratio of alginate immobilized cells is studied to reduce the water volume in desulfurization systems. Long-term recycling of BDS by alginate immobilized cells is carried out with oil/water ratio at 5 ： 1. The immobilized cells are successfully reused over 15 batch cycles. In the last batch, the desulfurization activity remains at least 75% of the first batch.Biodesulfurization(BDS) is a promising technology for deep desulfurization.In this work,Pseudomo-nas delafieldii R-8 cells are immobilized in calcium alginate beads and used for BDS of transportation fuels.It is found that thiophene and dibenzothiophene(DBT) can be simultaneously metabolized by immobilized R-8 cells.The initial sulfur content in the model oil is 300 mg?kg?1(thiophene︰DBT 1︰1).After 10 h of treatment,the thiophene concentration is reduced by 40%,while DBT is reduced by 25%.The utilization rate of thiophene is faster than that of DBT.Moreover,the oil/water ratio of alginate immobilized cells is studied to reduce the water volume in desulfurization systems.Long-term recycling of BDS by alginate immobilized cells is carried out with oil/water ratio at 5︰1.The immobilized cells are successfully reused over 15 batch cycles.In the last batch,the desulfurization activity remains at least 75% of the first batch.

关 键 词：BIODESULFURIZATION simultaneous removal alginate immobilization long-term recycling 

分 类 号：TE624.1[石油与天然气工程—油气加工工程]