COMT活性与子宫内膜癌发生和发展相关  

Association between catechol-O-methyltransferase activity and the development and progression of endometrial cancer

作　　者：李方[1] 付焱[2] 李俊玉[1] 刘雪静[1] 李利[1] 

机构地区：[1]河北医科大学第四医院妇产科,石家庄050011 [2]河北医科大学药学院新药开发研究室,石家庄050017

出　　处：《肿瘤》2012年第2期119-123,共5页Tumor

基　　金：河北省自然科学基金资助项目(编号:C2006000857);河北省教育厅高校强势特色学科资助项目[编号:冀教高(2005)52号]

摘　　要：目的:探讨儿茶酚氧位甲基转移酶(catechol-O-methyltransferase,COMT)的活性与子宫内膜癌发生、发展的关系。方法:建立体外反应体系,以3,4-二羟基苯甲酸(3,4-dihydroxybenzoic acid,DBA)为底物,采用反相高效液相色谱法检测终反应产物4-羟基-3-甲氧基苯甲酸(4-hydroxy-3-methoxybenzoic acid,4-OH-3-MBA)的浓度,以此反映COMT的活性。应用此方法检测子宫内膜癌组织、正常子宫内膜组织(对照组)及待检者外周血中COMT活性,并比较子宫内膜癌组和对照组患者中COMT的活性差异。结果:可溶性COMT(soluble-COMT,S-COMT)是子宫内膜组织中COMT的主要活性形式,子宫内膜组织中S-COMT活性明显高于外周血中S-COMT的活性(P=0.000);子宫内膜癌组织中S-COMT的活性明显低于对照组,差异有统计学意义(P=0.001)。低分化的子宫内膜癌组织中S-COMT的活性明显低于高、中分化的子宫内膜癌组织,差异有统计学意义(P=0.042)。结论:人子宫内膜组织中COMT的活性改变可能与子宫内膜癌的发生、发展有关。Objective：To investigate the association between the catechol-O-methyltransferase（COMT） activity and the development and progression of endometrial cancer.Methods：The in vitro reaction system was established and the 3,4-dihydroxybenzoic acid（DBA） was used as a substrate.The concentration of final product 4-hydroxy-3-methoxybenzoic acid（4-OH-3-MBA） which indicated the activity of COMT was detected by reversed-phase high-performance liquid chromatography（RP-HPLC）.The COMT activity in the endometrial cancer tissues and the normal endometrial tissues（as the control） was examined and compared,and the COMT activity in the peripheral blood was also detected.Results：The essential biologically active form of COMT was soluble-COMT（S-COMT） in the endometrial tissues.The S-COMT activity level was higher in the endometrial tissues than that in the peripheral blood（P = 0.000）,but it was highest in the normal endometrial tissues（P = 0.001）.The S-COMT activity level in the poorly differentiated endometrial cancer tissues was lower than those in the moderately and well differentiated endometrial cancer tissues（P = 0.042）.Conclusion：The variation in COMT activity level in the endometrial tissues is associated with the development and progression of endometrial cancer.

关 键 词：子宫内膜肿瘤 组织 血液 儿茶酚氧位甲基转移酶 

分 类 号：R169.41[医药卫生—公共卫生与预防医学]