包裹液态氟碳的高分子微球增强高强度聚焦超声消融兔肝的实验研究  

Experimental study of polymeric microcapsules with liquid perfluorocarbon enhancing high intensity focused ultrasound ablation of normal liver

作　　者：沈红霞[1] 郑元义[1] 王志刚[1] 周洋[1] 孙阳[1] 王琦[1] 李宜蓁[1] 

机构地区：[1]重庆医科大学超声影像学研究所重庆医科大学附属第二医院超声科,400010

出　　处：《中华超声影像学杂志》2012年第2期167-170,共4页Chinese Journal of Ultrasonography

基　　金：国家自然科学基金重点项目（81130025）;国家重点基础研究发展973计划（2011CB707900）;国家自然科学基金面上项目（30770566,30900371）;重庆市重点攻关项目（2010AB50952）;重庆高校优秀成果转化项目（Kjzh10201）

摘　　要：目的 探讨自制包裹液态氟碳的高分子微球增强高强度聚焦超声（HIFU）体外消融正常牛肝的最佳功率及体内消融正常兔肝的疗效.方法 实验分为两部分：第一部分为HIFU消融离体牛肝实验,分别注射四种不同的药物后行HIFU消融,寻找最佳消融功率与时间.实验分为4组：1组,HIFU＋生理盐水（NACL）;2组,HIFU＋乳酸/羟基乙酸共聚物（PLGA）；3组,HIFU＋液态氟碳（PFH）；4组,HIFU＋ PLGA＋ PFH.第二部分应用HIFU消融正常兔肝,同样分为以上4组,分别经兔耳缘静脉注射药物后以相同的参数行HIFU消融,观测并相互比较各组靶区消融前后超声声像图面积、灰阶值及实际肝损伤情况.结果 离体牛肝实验中2、3、4组均在150 W、2 s出现声像图灰度的变化和肝的凝固性坏死.正常兔肝实验中,3、4组靶区的平均超声灰阶值与面积大于1、2组,差异有统计学意义（P ＜0.001）,且随时间延长其灰阶值与面积逐渐缩小,而3、4组间比较差异无统计学意义（P＞0.05）；病理检查1、2组肝组织未见明显损伤,3、4组靶区肝组织见出血性坏死或白色凝固性坏死.结论 包裹液态氟碳的高分子微球可有效增强HIFU的消融效率,为一种较好的HIFU增效剂.Objective To study the optimal power of self-made polymeric microcapsules with liquid perfluorocarbon enhancing high intensity focused ultrasound （HIFU） ablation of normal bovine in vitro and the treatment efficacy of HIFU ablation of normal rabbit liver in vivo.Methods The experiment was divided into two parts：the first part was HIFU ablation of bovine in vitro：The bovine was injected different drugs before HIFU ablation to find the optimal power and treatment time.It was divided into four groups：①HIFU ＋ 0.9% NaCl（NACL） group; （② HIFU ＋ poly（ lactic-co-glycolic acid） （PLGA） group; ③ HIFU ＋ perfluorohexane（PFH） group; ④ HIFU ＋ PLGA ＋ PFH group; The second part was HIFU ablation of normal rabbit liver.Similarly,It was also divided into four groups as above.Each group was undergone HIFU ablation with same parameters after ear vein injection of drug.Then the ultrasonographic changes and its value,and the actual situation of liver damage in target areas of each group were observed and compared before and after the ablation area.Results In experiments of vitro bovine,gray scale changes and coagulation necrosis could be seen in the liver in group 2,3,4 with HIFU power of 150 W and treatment time of 2 s.In experiment of normal rabbit liver,the average gray-scale of group 3 and 4 were larger than that in group 1 and 2,the difference was statistically significant （P 〈0.001）,and its gray change area gradually reduced with time elapsed.While there was no statistically significant difference between group 3 and 4 （ P 〉0.05）.In pathological specimens,there was no significant liver damage in group 1 and 2.There was hemorrhagic necrosis and white coagulation necrosis in group 3 and 4.Conclusions The polymeric microcapsules with liquid perfluorocarbon can effectively enhance the efficacy of HIFU ablation and be used as a good synergist for HIFU.

关 键 词：超声 高强聚焦 肝 

分 类 号：R445.1[医药卫生—影像医学与核医学]