水飞蓟宾对D-半乳糖诱导衰老大鼠抑制糖氧化应激反应及改善学习记忆作用  

Effects of silybin on inhibiting glycoxidative stress and improving the learning and memory in aging rats treated by D-galactose

作　　者：傅永锦[1] 潘竞锵 张小牧[1] 邝少松[3] 

机构地区：[1]广州市中医医院 [2]广州市中医中药研究所 [3]广东省医学实验动物中心

出　　处：《中国临床药理学与治疗学》2012年第1期10-14,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:观察水飞蓟宾对D-半乳糖(D-gal)诱导衰老大鼠糖化氧化应激反应及学习记忆作用的影响。方法:采用D-gal诱导衰老大鼠模型;采用Morris水迷宫测定法检测大鼠学习记忆能力。实验结束后测定大鼠血浆糖化血红蛋白(HbA1c)、血浆非酶糖基化终产物(AGEs)和果糖胺(FRA)含量、红细胞醛糖还原酶(AR)活性及脑组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)、AGEs含量。结果:水飞蓟宾150mg/kg和75mg/kg均能明显抑制D-gal诱导的AR活性增高(P<0.01),降低FRA、HbA1c和AGEs含量(P<0.01或P<0.05),能不同程度地降低大鼠脑组织AGEs和MDA含量,提高SOD活性(P<0.01)。结论:水飞蓟宾对D-半乳糖所致衰老大鼠学习记忆障碍具有保护作用,能增强学习记忆能力,其机制可能与抑制糖氧化应激反应有关。ABSTRACT AIM： To investigate the effects of silybin on the glycoxidative stress and the abili- ties of learning and memory in aging rats induced by D-galactose. METHODS： The aging rats in- duced by D-galactose, the abilities of learning and memory were measured by Morris water maze test in rats. To assess the glycoxidative stress, the glycosylated hemoglobin （HbAlc） and fruetosamine （FRA）, advanced glycation end products（AGEs） in plasma, the activity of aldose reductase（AR） in red blood cell （RBC） were used , the levels of malondialdehyde （MDA） and AGEs and ability of superoxide dismutase（SOD） in brain tissue were also acquired. RESULTS：Compared with the control group, Si-ly groups（150 mg/kg and 75 mg/kg ） obviously inhibited the activity of AR（P〈0.01）, reduced the levels of FRA, HbAlc and AGEs in rats treated by D-galactose（P〈0.01 or P〈0.05）, and the levels of MDA and AGEs were reduced while the activity of SOD in brain were elevated （P〈0.01 ）. CONCLUSION. Silybin could im- prove the abilities of learning and memory in rats treated by D-galactose, and it might be related to the inhibition of glycoxidative stress and ability of AR.

关 键 词：水飞蓟宾 衰老 D-半乳糖 糖氧化应激 反应 学习记忆 

分 类 号：R965.2[医药卫生—药理学]